BRENDA - Enzyme Database
show all sequences of 1.5.1.22

Strombine [N-(carboxymethyl)-D-alanine]dehydrogenase and alanopine [meso-N-(1-carboxyethyl)-alanine]dehydrogenase from the mussel Mytilus edulis L.

Dando, P.R.; Biochem. Soc. Trans. 9, 297-298 (1981)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
40
-
L-Ala
Gly
Mytilus edulis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mytilus edulis
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38200
-
gel filtration
Mytilus edulis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mytilus edulis
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Mytilus edulis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adductor
-
Mytilus edulis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
r
13369
Mytilus edulis
D-Strombine + NAD+ + H2O
-
13369
Mytilus edulis
-
L-Ala + pyruvate + NADH
r
13369
Mytilus edulis
meso-Alanopine + NAD+ + H2O
-
13369
Mytilus edulis
-
L-Ala + pyruvate + NADH
in the reverse reaction: 81% of the activity with meso-alanopine compared to strombine
13369
Mytilus edulis
meso-Alanopine + NAD+ + H2O
-
13369
Mytilus edulis
-
L-Met + pyruvate + NADH
3.7% of the activity with Gly
13369
Mytilus edulis
?
-
-
-
-
L-Ser + pyruvate + NADH
31% of the activity with Gly
13369
Mytilus edulis
?
-
-
-
-
L-Thr + pyruvate + NADH
3.7% of the activity with Gly
13369
Mytilus edulis
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
with Gly and pyruvate as substrates
Mytilus edulis
9
-
with strombine as substrate
Mytilus edulis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Mytilus edulis
NADH
no activity with NADPH
Mytilus edulis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Mytilus edulis
NADH
no activity with NADPH
Mytilus edulis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
40
-
L-Ala
Gly
Mytilus edulis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mytilus edulis
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38200
-
gel filtration
Mytilus edulis
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Mytilus edulis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adductor
-
Mytilus edulis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
r
13369
Mytilus edulis
D-Strombine + NAD+ + H2O
-
13369
Mytilus edulis
-
L-Ala + pyruvate + NADH
r
13369
Mytilus edulis
meso-Alanopine + NAD+ + H2O
-
13369
Mytilus edulis
-
L-Ala + pyruvate + NADH
in the reverse reaction: 81% of the activity with meso-alanopine compared to strombine
13369
Mytilus edulis
meso-Alanopine + NAD+ + H2O
-
13369
Mytilus edulis
-
L-Met + pyruvate + NADH
3.7% of the activity with Gly
13369
Mytilus edulis
?
-
-
-
-
L-Ser + pyruvate + NADH
31% of the activity with Gly
13369
Mytilus edulis
?
-
-
-
-
L-Thr + pyruvate + NADH
3.7% of the activity with Gly
13369
Mytilus edulis
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
with Gly and pyruvate as substrates
Mytilus edulis
9
-
with strombine as substrate
Mytilus edulis
Other publictions for EC 1.5.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
-
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
711788
Lee
Purification and kinetic chara ...
Meretrix lusoria
Comp. Biochem. Physiol. B
158
38-45
2010
-
-
-
-
-
-
6
7
-
6
1
-
-
2
-
-
1
-
-
2
-
1
6
1
1
1
1
-
1
1
-
-
12
-
-
-
-
-
-
-
-
-
-
6
12
7
-
6
1
-
-
-
-
1
-
2
-
1
6
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
703299
Plese
Strombine dehydrogenase in the ...
Suberites domuncula
Comp. Biochem. Physiol. B
154
102-107
2009
-
-
-
-
-
-
1
1
-
-
2
-
-
3
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686211
Endo
-
Purification, characterization ...
Marphysa sanguinea
Comp. Biochem. Physiol. B
147B
293-307
2007
-
-
1
-
-
-
-
13
-
-
3
-
-
1
-
-
1
-
-
1
2
-
13
-
-
-
-
15
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
13
-
-
3
-
-
-
-
1
-
1
2
-
13
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
688240
Quiroga
-
Aerobic and anaerobic metaboli ...
Paraprionospio pinnata
J. Mar. Biolog. Assoc. UK
87
459-463
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Asterias amurensis, Azumapecten farreri nipponensis, Balanus cariosus, Buccinum isaotakii, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Halichondria japonica, Haliotis discus hannai, Hemigrapsus sanguineus, Littorina brevicula, Meretrix lusoria, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Aurelia aurita, no activity in Cellana grata, no activity in Halocynthia roretzi, no activity in Hexagrammos otakii, no activity in Liolophura japonica, no activity in Loligo bleekeri, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, no activity in Todarodes pacificus, Octopus vulgaris, Pagurus samuelis, Patiria pectinifera, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
54
-
-
-
-
-
-
54
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
1
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
8
-
-
-
1
-
1
-
-
1
-
-
1
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
8
-
-
-
1
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
3
13
-
-
1
-
-
1
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
13
-
-
1
-
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
1
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-