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Literature summary for 1.5.1.21 extracted from
- The putative malate/lactate dehydrogenase from Pseudomonas putida is an NADPH-dependent DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase involved in the catabolism of D-lysine and D-proline (2005), J. Biol. Chem., 280, 5329-5335.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, overexpression in Escherichia coli | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | dpk disruption mutant is unable to grow on D-lysine or D-proline as sole carbon source | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | inhibits the reverse reaction | Pseudomonas putida |  |
| pyrroline-2-carboxylate | inhibits the reverse reaction | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Pseudomonas putida | |
| 0.034 | - |
NADP+ | pH 10.0, 35°C, with substrate L-proline | Pseudomonas putida | |
| 0.034 | - |
NADPH | pH 8.0, 35°C, with substrate DELTA1-pyrroline-2-carboxylate | Pseudomonas putida | |
| 0.14 | - |
NADP+ | pH 10.0, 35°C, with substrate L-pipecolate | Pseudomonas putida | |
| 0.14 | - |
NADPH | pH 8.0, 35°C, with substrate DELTA1-piperideine-2-carboxylate | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas putida | enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview | ? | - |
? | |
| additional information | Pseudomonas putida ATCC 12633 | enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q5FB93 | strain ATCC12633, gene dpkA | - |
| Pseudomonas putida ATCC 12633 | Q5FB93 | strain ATCC12633, gene dpkA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli, to homogeneity | Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | specificity for carbon sources, wild-type shows the highest growth rate on D-proline, the deletion mutant on L-lysine, overview | Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
| 0.33 | - |
purified recombinant enzyme, substrates DELTA1-pyrroline-2-carboxylate and NADH | Pseudomonas putida |
| 0.67 | - |
purified recombinant enzyme, substrates DELTA1-piperideine-2-carboxylate and NADH | Pseudomonas putida |
| 1.4 | - |
purified recombinant enzyme, substrates L-pipecolate and NADP+ | Pseudomonas putida |
| 51 | - |
purified recombinant enzyme, substrates DELTA1-pyrroline-2-carboxylate and NADPH | Pseudomonas putida |
| 92 | - |
purified recombinant enzyme, substrates DELTA1-piperideine-2-carboxylate and NADPH | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DELTA1-piperideine-2-carboxylate + NADPH | NADH is a poor electron donor, lower activity in the reverse reaction direction | Pseudomonas putida | L-pipecolate + NADP+ | - |
r | |
| DELTA1-piperideine-2-carboxylate + NADPH | NADH is a poor electron donor, lower activity in the reverse reaction direction | Pseudomonas putida ATCC 12633 | L-pipecolate + NADP+ | - |
r | |
| DELTA1-pyrroline-2-carboxylate + NADPH | NADH is a poor electron donor, lower activity in the reverse reaction direction | Pseudomonas putida | L-proline + NADP+ | - |
r | |
| DELTA1-pyrroline-2-carboxylate + NADPH | NADH is a poor electron donor, lower activity in the reverse reaction direction | Pseudomonas putida ATCC 12633 | L-proline + NADP+ | - |
r | |
| additional information | enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview | Pseudomonas putida | ? | - |
? | |
| additional information | substrate specificity, enzyme shows no malate or lactate dehydrogenase activity | Pseudomonas putida | ? | - |
? | |
| additional information | enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview | Pseudomonas putida ATCC 12633 | ? | - |
? | |
| additional information | substrate specificity, enzyme shows no malate or lactate dehydrogenase activity | Pseudomonas putida ATCC 12633 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at, forward reaction | Pseudomonas putida |
| 10 | - |
assay at, reverse reaction | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | dependent on | Pseudomonas putida | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | substrate and product inhibition kinetics, overview | Pseudomonas putida |
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