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Literature summary for 1.5.1.20 extracted from
- Unique holoenzyme dimers of the tetrameric enzyme Escherichia coli methylenetetrahydrofolate reductase: characterization of structural features associated with modulation of the enzyme's function (2003), Biochemistry, 42, 3921-3928.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in strain BL21(DE3) | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
dimeric enzyme, gel filtration | Escherichia coli |
| 133000 | - |
tetrameric enzyme, gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from strain BL21(DE3) | Escherichia coli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| dissociated enzyme after treatment with 1 M urea, no refolding and renaturation is possible after treatment with 1.2 M NaCl | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methyltetrahydrofolate + NADP+ | - |
Escherichia coli | 5,10-methylenetetrahydrofolate + NADPH | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | smallest functional unit of the enzyme | Escherichia coli |
| More | alterations in the hydrophobic interactions by 1 M urea lead to dissociation of the native tetramer, resulting in stabilization of enzymatically active holoenzyme dimers, at 3 M urea followed by unfolding of the dimers to denatured monomers along with dissociation of FAD from the enzyme subunits, alterations of the electrostatic interactions by 1.2 M NaCl lead to dissociation of the enzyme into inactive, partially denatured dimers | Escherichia coli |
| tetramer | composed of 2 active dimers | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5,10-methylenetetrahydrofolate reductase (FADH2) | - |
Escherichia coli |
| 5-methylenetetrahydrofolate:NADP+ oxidoreductase | - |
Escherichia coli |
| 5-methyltetrahydrofolate:(acceptor) oxidoreductase | - |
Escherichia coli |
| methylenetetrahydrofolate reductase | - |
Escherichia coli |
| methylenetetrahydrofolate reductase (NADPH) | - |
Escherichia coli |
| More | EC 1.7.99.5 included with EC 1.5.1.20 | Escherichia coli |
| MTHFR | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
dimeric enzyme, 50% inactivatin at pH 7.2 | Escherichia coli |
| 58 | - |
tetrameric enzyme, 50% inactivatin at pH 7.2 | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | enzyme-bound | Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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