Any feedback?
Literature summary for 1.5.1.20 extracted from
- Feedback inhibition of methylene-tetrahydrofolate reductase in rat liver by S-adenosylmethionine (1967), Biochim. Biophys. Acta, 139, 217-220.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosylmethionine | inhibitory effect at a physiological concentration, dependent on incubation temperature, binds to an allosteric regulatory site different from catalytic site, reversed by S-adenosylhomocysteine; in vivo feedback-inhibition | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | 0.08 | 5-methyltetrahydrofolate | fresh extract | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADH | Rattus norvegicus | physiological direction: 5-methyltetrahydrofolate formation, inhibition by S-adenosylmethionine functions as a feedback-type metabolic regulation in vivo | 5-methyltetrahydrofolate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADH | physiological direction: 5-methyltetrahydrofolate formation, inhibition by S-adenosylmethionine functions as a feedback-type metabolic regulation in vivo | Rattus norvegicus | 5-methyltetrahydrofolate + NAD+ | - |
? | |
| 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
| 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: NADH as reduced acceptor | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
S-adenosylmethionine | - |
Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
