General Stability | Organism |
---|---|
extremely sensitive to proteolysis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosylmethionine | inhibition of the enzyme in crude extracts from fresh liver biopsies, but not after purification from cadaver liver | Homo sapiens | |
tetrahydrofolate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
5,10-methylenetetrahydrofolate | NADPH-5,10-methylenetetrahydrofolate-oxidoreductase reaction | Homo sapiens | |
0.017 | - |
NADPH | NADPH-menadione oxidoreductase reaction | Homo sapiens | |
0.038 | - |
5-methyltetrahydrofolate | 5,10-methylenetetrahydrofolate-oxidoreductase reaction | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
SDS-PAGE | Homo sapiens |
150000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADPH | Homo sapiens | first step in biosynthesis of methyl groups | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.367 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADPH | - |
Homo sapiens | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | first step in biosynthesis of methyl groups | Homo sapiens | ? | - |
? | |
5-methyltetrahydrofolate + oxidized menadione | - |
Homo sapiens | 5,10-methylenetetrahydrofolate + reduced menadione | - |
? | |
NADPH + H+ + menadione | - |
Homo sapiens | NADP+ + menadiol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000, SDS-PAGE and inhibition studies | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
NADPH-tetrahydrofolate oxidoreductase activity | Homo sapiens |
6.6 | - |
methyltetrahydrofolate-menadione oxidoreductase activity | Homo sapiens |
7.2 | - |
NADPH-menadione oxidoreductase activity | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required | Homo sapiens |