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Literature summary for 1.5.1.19 extracted from
- Purification and characterization of the crown gall specific enzyme nopaline synthase (1979), Biochemistry, 18, 3755-3760.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | substrate inhibition above 10fold Km | Helianthus annuus |  |
| NADPH | substrate inhibition above 10fold Km | Helianthus annuus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
NADPH | nopaline synthesis | Helianthus annuus | |
| 0.009 | - |
NADPH | ornaline synthesis | Helianthus annuus | |
| 0.74 | - |
L-arginine | - |
Helianthus annuus | |
| 0.9 | - |
2-oxoglutarate | - |
Helianthus annuus | |
| 1 | - |
NADPH | - |
Helianthus annuus | |
| 3.7 | - |
L-ornithine | - |
Helianthus annuus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, SDS-PAGE | Helianthus annuus |
| 158000 | - |
crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37, gel filtration | Helianthus annuus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + 2-oxoglutarate + NADPH | Helianthus annuus | in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues | N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | - |
? | |
| L-ornithine + 2-oxoglutarate + NADPH | Helianthus annuus | in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues | N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
sunflower, crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37 | Helianthus annuus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+ | ter-bi rapid-equilibrium random-order mechanism | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| crown gall | induced by Agrobacterium tumefaciens, strain C58 or T37 in Helianthus annuus L. | Helianthus annuus | - |
| additional information | no activity in habituated tissue or in octopine-type crown gall tissue | Helianthus annuus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.34 | - |
- |
Helianthus annuus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, several months in 10 mM Tris | Helianthus annuus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + 2-oxoglutarate + NADPH | - |
Helianthus annuus | N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | i.e. nopaline | ? | |
| L-arginine + 2-oxoglutarate + NADPH | in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues | Helianthus annuus | N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | - |
? | |
| L-ornithine + 2-oxoglutarate + NADPH | - |
Helianthus annuus | N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O | i.e. ornaline | ? | |
| L-ornithine + 2-oxoglutarate + NADPH | in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues | Helianthus annuus | N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Helianthus annuus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Helianthus annuus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
nopaline synthesis | Helianthus annuus |
| 6.5 | - |
ornaline synthesis | Helianthus annuus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Helianthus annuus | |
| NADPH | - |
Helianthus annuus | |
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