General Stability | Organism |
---|---|
enzyme is sensitive to inactivation by dilution | Arthrobacter globiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-ephedrine | substrate inhibition at higher concentrations | Arthrobacter globiformis | |
additional information | EDTA causes no remarkable loss of activity | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(-)-ephedrine | - |
Arthrobacter globiformis | |
0.11 | - |
NAD+ | - |
Arthrobacter globiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-ephedrine + NAD+ | Arthrobacter globiformis | first step in the catabolism of (-)-ephedrine | (R)-2-methylimino-1-phenylpropan-1-ol + NADH | - |
? | |
(-)-ephedrine + NAD+ | Arthrobacter globiformis | plays role in the metabolism of quite a number of different aromatic and aliphatic amino alcohols | (R)-2-methylimino-1-phenylpropan-1-ol + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, 9.1fold enrichment | Arthrobacter globiformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
additional information | Arthrobacter globiformis |
0.61 | - |
- |
Arthrobacter globiformis |
Storage Stability | Organism |
---|---|
-20°C, crude extract, 6 months, no appreciable loss of activity | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-norephedrine + NAD+ | higher rate than (-)-ephedrine | Arthrobacter globiformis | 2-imino-1-hydroxy-1-phenylpropane + NADH | - |
? | |
(+)-norephedrine + NAD+ | norephedrine is identical with threo-2-amino-1-hydroxy-1-phenylpropane | Arthrobacter globiformis | 2-imino-1-hydroxy-1-phenylpropane + NADH | - |
? | |
(+)-pseudoephedrine + NAD+ | higher rate than (-)-ephedrine | Arthrobacter globiformis | 2-methylimino-1-phenylpropan-1-ol + NADH | - |
? | |
(+)-pseudoephedrine + NAD+ | pseudoephedrine is identical with DL-threo-2-(methylamino)-1-phenylpropan-1-ol | Arthrobacter globiformis | 2-methylimino-1-phenylpropan-1-ol + NADH | - |
? | |
(-)-ephedrine + NAD+ | (-)-ephedrine is identical with 2-methylamino-1-phenyl-1-propanal | Arthrobacter globiformis | (R)-2-methylimino-1-phenylpropan-1-ol + NADH | phenylacetylcarbinol is identical with 1-hydroxy-1-phenylpropan-2-one | ? | |
(-)-ephedrine + NAD+ | (-)-ephedrine is identical with 2-methylamino-1-phenyl-1-propanal | Arthrobacter globiformis | (R)-2-methylimino-1-phenylpropan-1-ol + NADH | three products: NADH, methylamine and phenylacetylcarbinol or its tautomeric form methylbenzoylcarbinol, ratio of NADH to methylamine is 1:1.1, enzyme attacks (-)-ephedrine at CH-NHCH3, yielding 2-methylimino-1-phenylpropanol, which is immediately hydrolyzed forming methylamine and phenylacetylcarbinol | ? | |
(-)-ephedrine + NAD+ | first step in the catabolism of (-)-ephedrine | Arthrobacter globiformis | (R)-2-methylimino-1-phenylpropan-1-ol + NADH | - |
? | |
(-)-ephedrine + NAD+ | plays role in the metabolism of quite a number of different aromatic and aliphatic amino alcohols | Arthrobacter globiformis | (R)-2-methylimino-1-phenylpropan-1-ol + NADH | - |
? | |
(-)-sympatol + NAD+ | higher rate than (-)-ephedrine | Arthrobacter globiformis | 4-[(1R,2E)-1-hydroxy-2-(methylimino)ethyl]phenol + NADH | - |
? | |
(-)-sympatol + NAD+ | sympatol is identical with p-hydroxy-alpha-[(methylamino)methyl]benzyl tartrate | Arthrobacter globiformis | 4-[(1R,2E)-1-hydroxy-2-(methylimino)ethyl]phenol + NADH | - |
? | |
additional information | wide range of ethanolamines as substrates, overview | Arthrobacter globiformis | ? | - |
? | |
suprifene + NAD+ | higher rate than (-)-ephedrine | Arthrobacter globiformis | p-hydroxy-alpha-[(1-methylimino)ethyl]benzyl alcohol + NADH | - |
? | |
suprifene + NAD+ | suprifene is identical with p-hydroxyephedrine | Arthrobacter globiformis | p-hydroxy-alpha-[(1-methylimino)ethyl]benzyl alcohol + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arthrobacter globiformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
2 min, complete loss of activity | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 11.5 | in 0.1 M glycine/NaOH buffer | Arthrobacter globiformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9.5 | 12.2 | pH 9.5: 60% of maximum activity, pH 12.2: 60% of maximum activity | Arthrobacter globiformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
24 h, 68% loss of activity | Arthrobacter globiformis |
7.2 | - |
24 h, 53% loss of activity | Arthrobacter globiformis |
7.5 | 8.5 | at 4°C maximum stability, after 24 h 20% loss of activity | Arthrobacter globiformis |
10 | - |
24 h, 67% loss of activity | Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required for enzyme activity, NAD+ can not be replaced by NADP+, FAD or FMN | Arthrobacter globiformis |