BRENDA - Enzyme Database
show all sequences of 1.5.1.17

Alanopine dehydrogenase: purification and characterization of the enzyme from Littorina littorea foot muscle

Plaxton, W.C.; Storey, K.B.; J. Comp. Physiol. 149, 57-65 (1982)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
200 mM, 16% inhibition
Littorina littorea
2-oxoglutarate
-
Littorina littorea
3-phosphoglycerate
-
Littorina littorea
ADP
competitive with respect to NADH
Littorina littorea
ATP
competitive with respect to NADH
Littorina littorea
citrate
-
Littorina littorea
D-strombine
-
Littorina littorea
dihydroxyacetone phosphate
-
Littorina littorea
fructose 1,6-bisphosphate
-
Littorina littorea
KCl
200 mM, 33% inhibition
Littorina littorea
L-Ala
substrate inhibition
Littorina littorea
L-Arg
-
Littorina littorea
L-Glu
-
Littorina littorea
L-Ile
-
Littorina littorea
L-lactate
-
Littorina littorea
L-Leu
-
Littorina littorea
L-Lys
-
Littorina littorea
meso-Alanopine
product inhibition
Littorina littorea
Mg2+
-
Littorina littorea
NaCl
200 mM, 38% inhibition
Littorina littorea
NAD+
product inhibition
Littorina littorea
NH4Cl
200 mM, 23% inhibition
Littorina littorea
oxamate
-
Littorina littorea
pyruvate
substrate inhibition
Littorina littorea
succinate
-
Littorina littorea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
-
pyruvate
at pH 6.5
Littorina littorea
0.18
-
NAD+
pH-independent
Littorina littorea
0.26
-
pyruvate
at pH 7.5
Littorina littorea
6.5
-
meso-Alanopine
at pH 6.5
Littorina littorea
9
-
NADH
pH-independent
Littorina littorea
14.9
-
L-Ala
at pH 6.5
Littorina littorea
23.8
-
L-Ala
at pH 7.5
Littorina littorea
50
-
Gly
meso-alanopine, at pH 8.5
Littorina littorea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42200
-
gel filtration
Littorina littorea
42400
-
1 * 42400, SDS-PAGE
Littorina littorea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Littorina littorea
the enzyme functions in cytoplasmic redox balance during anoxia stress
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Littorina littorea
-
-
-
Purification (Commentary)
Commentary
Organism
-
Littorina littorea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
foot muscle
-
Littorina littorea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
240
-
-
Littorina littorea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
-
13374
Littorina littorea
Strombine + NAD+ + H2O
-
-
-
-
L-2-Aminobutyrate + pyruvate + NADH
-
13374
Littorina littorea
?
-
-
-
-
L-Ala + 2-oxobutanoate + NADH
-
13374
Littorina littorea
?
-
-
-
-
L-Ala + glyoxylate + NADH
-
13374
Littorina littorea
?
-
-
-
-
L-Ala + oxaloacetate + NADH
-
13374
Littorina littorea
?
-
-
-
-
L-Ala + pyruvate + NADH
-
13374
Littorina littorea
2,2'-Iminodipropanoate + NAD+ + H2O
-
-
-
-
L-Ser + pyruvate + NADH
-
13374
Littorina littorea
?
-
-
-
-
additional information
D-strombine is not oxidized
13374
Littorina littorea
?
-
-
-
-
additional information
the enzyme functions in cytoplasmic redox balance during anoxia stress
13374
Littorina littorea
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 42400, SDS-PAGE
Littorina littorea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
with 100 mM L-Ala, 0.1 mM NADH and 1 mM pyruvate as substrates
Littorina littorea
7.5
-
with 130 mM L-Ala, 0.1 mM NADH and 1.3 mM pyruvate as substrates
Littorina littorea
8.5
-
with 10 mM meso-alanopine as substrate
Littorina littorea
9.2
-
with 50 mM meso-alanopine as substrate
Littorina littorea
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Littorina littorea
NADH
no activity with NADPH
Littorina littorea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Littorina littorea
NADH
no activity with NADPH
Littorina littorea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
200 mM, 16% inhibition
Littorina littorea
2-oxoglutarate
-
Littorina littorea
3-phosphoglycerate
-
Littorina littorea
ADP
competitive with respect to NADH
Littorina littorea
ATP
competitive with respect to NADH
Littorina littorea
citrate
-
Littorina littorea
D-strombine
-
Littorina littorea
dihydroxyacetone phosphate
-
Littorina littorea
fructose 1,6-bisphosphate
-
Littorina littorea
KCl
200 mM, 33% inhibition
Littorina littorea
L-Ala
substrate inhibition
Littorina littorea
L-Arg
-
Littorina littorea
L-Glu
-
Littorina littorea
L-Ile
-
Littorina littorea
L-lactate
-
Littorina littorea
L-Leu
-
Littorina littorea
L-Lys
-
Littorina littorea
meso-Alanopine
product inhibition
Littorina littorea
Mg2+
-
Littorina littorea
NaCl
200 mM, 38% inhibition
Littorina littorea
NAD+
product inhibition
Littorina littorea
NH4Cl
200 mM, 23% inhibition
Littorina littorea
oxamate
-
Littorina littorea
pyruvate
substrate inhibition
Littorina littorea
succinate
-
Littorina littorea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
-
pyruvate
at pH 6.5
Littorina littorea
0.18
-
NAD+
pH-independent
Littorina littorea
0.26
-
pyruvate
at pH 7.5
Littorina littorea
6.5
-
meso-Alanopine
at pH 6.5
Littorina littorea
9
-
NADH
pH-independent
Littorina littorea
14.9
-
L-Ala
at pH 6.5
Littorina littorea
23.8
-
L-Ala
at pH 7.5
Littorina littorea
50
-
Gly
meso-alanopine, at pH 8.5
Littorina littorea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42200
-
gel filtration
Littorina littorea
42400
-
1 * 42400, SDS-PAGE
Littorina littorea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Littorina littorea
the enzyme functions in cytoplasmic redox balance during anoxia stress
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Littorina littorea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
foot muscle
-
Littorina littorea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
240
-
-
Littorina littorea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
-
13374
Littorina littorea
Strombine + NAD+ + H2O
-
-
-
-
L-2-Aminobutyrate + pyruvate + NADH
-
13374
Littorina littorea
?
-
-
-
-
L-Ala + 2-oxobutanoate + NADH
-
13374
Littorina littorea
?
-
-
-
-
L-Ala + glyoxylate + NADH
-
13374
Littorina littorea
?
-
-
-
-
L-Ala + oxaloacetate + NADH
-
13374
Littorina littorea
?
-
-
-
-
L-Ala + pyruvate + NADH
-
13374
Littorina littorea
2,2'-Iminodipropanoate + NAD+ + H2O
-
-
-
-
L-Ser + pyruvate + NADH
-
13374
Littorina littorea
?
-
-
-
-
additional information
D-strombine is not oxidized
13374
Littorina littorea
?
-
-
-
-
additional information
the enzyme functions in cytoplasmic redox balance during anoxia stress
13374
Littorina littorea
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 42400, SDS-PAGE
Littorina littorea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
with 100 mM L-Ala, 0.1 mM NADH and 1 mM pyruvate as substrates
Littorina littorea
7.5
-
with 130 mM L-Ala, 0.1 mM NADH and 1.3 mM pyruvate as substrates
Littorina littorea
8.5
-
with 10 mM meso-alanopine as substrate
Littorina littorea
9.2
-
with 50 mM meso-alanopine as substrate
Littorina littorea
Other publictions for EC 1.5.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
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-
-
1
-
-
1
-
1
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-
-
1
-
-
2
-
-
-
1
1
-
2
-
-
-
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-
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-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
5
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
742962
Gohlke
Binding region of alanopine d ...
Arenicola marina
J. Chem. Inf. Model.
53
2493-2498
2013
-
-
-
-
-
-
1
3
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
1
-
-
-
2
-
-
-
3
1
-
-
-
-
-
3
-
-
-
-
1
1
3
-
-
-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
2
-
-
-
-
-
3
3
-
-
-
13290
Carvajal
-
Lactate dehydrogenase, alanopi ...
Concholepas concholepas
Comp. Biochem. Physiol. B
108
543-550
1994
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Azumapecten farreri nipponensis, Buccinum isaotakii, Cellana grata, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Haliotis discus hannai, Heterololigo bleekeri, Liolophura japonica, Littorina brevicula, Meretrix lusoria, Mesocentrotus nudus, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Asterias amurensis, no activity in Asterina pectinifera, no activity in Aurelia aurita, no activity in Balanus cariosus, no activity in Halichondria japonica, no activity in Halocynthia roretzi, no activity in Hemigrapsus sanguineus, no activity in Hexagrammos otakii, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pagurus samuelis, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, Octopus vulgaris, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Todarodes pacificus, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
11
-
-
-
1
-
1
-
-
1
-
-
2
-
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
11
-
-
-
1
-
-
-
1
-
2
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13366
Manchenko
Nonfluorescent negative stain ...
Polydora ciliata, Polydora commensalis, Polydora glycymerica, Pseudopolydora paucibranchiata
Anal. Biochem.
145
308-310
1985
-
4
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13368
Siegmund
Determination of meso-alanopin ...
Arenicola marina, Crassostrea angulata, Mytilus edulis, Nucula nitida
Hoppe-Seyler's Z. Physiol. Chem.
364
807-812
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
6
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13372
Plaxton
-
Purification and properties of ...
Busycotypus canaliculatum
Comp. Biochem. Physiol. B
76
321-326
1983
-
-
-
-
-
-
4
1
-
-
-
2
-
1
-
-
1
-
-
3
-
-
3
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
1
-
-
-
2
-
-
-
1
-
3
-
-
3
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
7
17
2
-
1
-
-
1
-
-
1
-
-
3
1
1
12
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
17
2
-
1
-
-
-
-
1
-
3
1
1
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13371
Plaxton
-
Tissue specific isozymes of al ...
Busycotypus canaliculatum
Can. J. Zool.
60
1568-1572
1982
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
7
-
-
2
-
-
-
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13374
Plaxton
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Alanopine dehydrogenase: purif ...
Littorina littorea
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57-65
1982
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25
8
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4
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1
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4
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13375
Baldwin
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The properties and functions o ...
Bursa sp., Cantharus undosus, Conomurex luhuanus, Conus arenatus, Cymatium pileare, Cypraea tigris, Cypraecassis rufa, Lambis lambis, Lambis millepeda, Lambis scorpius, Menathais tuberosa, Mitra eremitarum, Nassarius coronatus, Nassarius glans particeps, Nerita atramentosa, Strombus aurisdianae, Strombus canarium, Strombus gibberulus, Strombus labiatus, Strombus lentiginosus, Strombus sinuatus, Tibia martinii, Xenophora crispa
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1982
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4
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23
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46
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13369
Dando
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Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
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1981
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1
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6
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13370
Fields
Purification and properties of ...
Crassostrea gigas
Eur. J. Biochem.
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615-621
1981
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7
10
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8
1
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13367
Fields
Alanopine and strombine are no ...
Crassostrea gigas
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1980
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