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Literature summary for 1.5.1.15 extracted from

  • Mackenzie, R.E.
    Mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase (1997), Methods Enzymol., 281, 171-177.
    View publication on PubMed
Search for more literature for 1.5.1.15

Activating Compound

Activating Compound Comment Organism Structure
phosphate
-
 Mus musculus
phosphate absolute requirement, phosphate can be replaced by its analog arsenate Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloned cDNA for the human enzyme expressed in Escherichia coli K-38 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 L-5,10-methylenetetrahydrofolate
-
 Homo sapiens
0.013
-
 NAD+
-
 Spodoptera frugiperda
0.019
-
 L-5,10-methylenetetrahydrofolate
-
 Mus musculus
0.03
-
 methenyltetrahydrofolate
-
 Spodoptera frugiperda
0.096
-
 methenyltetrahydrofolate
-
 Homo sapiens
0.111
-
 NAD+
-
 Mus musculus
0.132
-
 NAD+
-
 Homo sapiens
0.155
-
 5,10-methenyltetrahydrofolate
-
 Mus musculus
0.17
-
 phosphate
-
 Mus musculus
0.19
-
 phosphate
-
 Homo sapiens
0.25
-
 Mg2+
-
 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Drosophila melanogaster 5737
-
cytoplasm
-
 Mus musculus 5737
-
cytoplasm
-
 Homo sapiens 5737
-
cytoplasm
-
 Saccharomyces cerevisiae 5737
-
cytoplasm
-
 Spodoptera frugiperda 5737
-
mitochondrion
-
 Drosophila melanogaster 5739
-
mitochondrion
-
 Mus musculus 5739
-
mitochondrion
-
 Homo sapiens 5739
-
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus
Mg2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
-
 Mus musculus
34000
-
-
 Homo sapiens
37000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH Drosophila melanogaster
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Homo sapiens
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Spodoptera frugiperda
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Drosophila melanogaster
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Homo sapiens
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Spodoptera frugiperda
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
 mouse, Ehrlich ascites tumor cells
-
Saccharomyces cerevisiae
-
-
-
Spodoptera frugiperda
-
 fall armyworm, cell line Sf9
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spodoptera frugiperda
from ascites tumor cells Mus musculus
recombinant enzyme, expressed in Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.9
-
-
 Mus musculus
40 44
-
 Homo sapiens
43
-
-
 Spodoptera frugiperda

Storage Stability

Storage Stability Organism
-80°C, enzyme is stable in 20-30% glycerol buffer and can be frozen for months without loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Drosophila melanogaster L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Homo sapiens L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Spodoptera frugiperda L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Drosophila melanogaster 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Homo sapiens 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Spodoptera frugiperda 5,10-methenyl-tetrahydrofolate + NADH
-
 r