Cloned (Comment) | Organism |
---|---|
gene ocs, DNA and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + pyruvate + NADH + H+ | Agrobacterium tumefaciens | - |
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O | - |
? | |
additional information | Agrobacterium tumefaciens | octopine [N2-(1-D-carboxyethyl)-L-arginine] is synthesized by a NAD(P)H-dependent soluble dehydrogenase that catalyzes the reductive condensation of pyruvate with L-arginine. Although the reaction may be reversible in vitro, the frequent use of the term synthase rather than dehydrogenase has emphasized the importance of biosynthesis, but not degradation, and distinguishes it from the mollusk octopine dehydrogenase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + pyruvate + NADH + H+ | - |
Agrobacterium tumefaciens | N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O | - |
? | |
L-arginine + pyruvate + NADH + H+ | the reduction reaction is highly preferred | Agrobacterium tumefaciens | N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O | - |
? | |
additional information | octopine [N2-(1-D-carboxyethyl)-L-arginine] is synthesized by a NAD(P)H-dependent soluble dehydrogenase that catalyzes the reductive condensation of pyruvate with L-arginine. Although the reaction may be reversible in vitro, the frequent use of the term synthase rather than dehydrogenase has emphasized the importance of biosynthesis, but not degradation, and distinguishes it from the mollusk octopine dehydrogenase | Agrobacterium tumefaciens | ? | - |
? | |
additional information | the enzyme OCS utilizes not only L-arginine, but also other amino acids, to yield the corresponding members of the octopine family. Analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+ | Agrobacterium tumefaciens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavin-containing opine dehydrogenase | - |
Agrobacterium tumefaciens |
OCS | - |
Agrobacterium tumefaciens |
Octopine synthase | - |
Agrobacterium tumefaciens |
pTi_008 | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Agrobacterium tumefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Agrobacterium tumefaciens |