Inhibitors | Comment | Organism | Structure |
---|---|---|---|
epsilon-aminohexanoate | - |
Helianthus annuus | |
NADP+ | uncompetitive with respect to pyruvate | Helianthus annuus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.85 | - |
L-Orn | - |
Helianthus annuus | |
8.8 | - |
L-Arg | - |
Helianthus annuus | |
11.2 | - |
L-Met | - |
Helianthus annuus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
1 * 38000, SDS-PAGE | Helianthus annuus |
39000 | - |
gel filtration | Helianthus annuus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus annuus | - |
crown-gall tumor-tissue induced by Agrobacterium tumefaciens | - |
Purification (Comment) | Organism |
---|---|
crown-gall tumor-tissue induced by Agrobacterium tumefaciens | Helianthus annuus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+ | partially ordered mechanism. NADPH binds first followed by the random order, rapid-equilibrium-binding of Arg and pyruvate. Orn probably binds before pyruvate | Helianthus annuus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
crown gall | induced by Agrobacterium tumefaciens | Helianthus annuus | - |
crown gall | the structural gene for octopine synthase is located in a central portion of the T-DNA of Agrobacterium tumefaciens Ti plasmid | Helianthus annuus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Helianthus annuus |
Storage Stability | Organism |
---|---|
-30°C, buffer containing glycerol, NADPH and 2-mercaptoethanol, stable for many weeks | Helianthus annuus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + pyruvate + NADH | - |
Helianthus annuus | N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O | - |
? | |
L-Arg + pyruvate + NADPH | - |
Helianthus annuus | N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O | - |
? | |
L-Gln + pyruvate + NADPH | - |
Helianthus annuus | N2-(D-1-Carboxyethyl)-L-Gln + NADP+ + H2O | - |
? | |
L-Lys + pyruvate + NADPH | - |
Helianthus annuus | Lysopine + NADP+ + H2O | - |
? | |
L-Met + pyruvate + NADPH | - |
Helianthus annuus | N2-(D-1-Carboxyethyl)-L-Met + NADP+ + H2O | - |
? | |
Orn + pyruvate + NADH | - |
Helianthus annuus | N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Helianthus annuus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Helianthus annuus | |
NADPH | - |
Helianthus annuus |