Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.1 extracted from

  • Watanabe, S.; Tanimoto, Y.; Yamauchi, S.; Tozawa, Y.; Sawayama, S.; Watanabe, Y.
    Identification and characterization of trans-3-hydroxy-l-proline dehydratase and DELTA1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria (2014), FEBS Open Bio, 4, 240-250 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene lhpI, DNA and amino acid sequence determination and analysis, phylogenetic tree, genetic organization of genes encoding enzyme involved in the trans-3-hydroxy-L-proline pathway and networks with D-lysine and D-proline, overview. Recombinant expression of His6-tagged enzyme in Escherichia coli Azospirillum brasilense

Protein Variants

Protein Variants Comment Organism
additional information generation of a lphI knockout mutant, AbLhpI-, the mutant strain is distinct from the wild-type strain in that trans-3-hydroxy-L-proline, D-proline, and D-lysine do not support growth as a sole carbon source, but there is no difference in growth on other carbon sources, including trans-4-hydroxy-L-proline, between the two strains Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.474
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
0.474
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
0.491
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
0.491
-
 DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
0.6
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
0.6
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
0.628
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
0.628
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
0.837
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
0.837
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
2.1
-
 (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
2.79
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
3.63
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
3.99
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
5.9
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
6.54
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
7.26
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
7.26
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
14.8
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
18.3
-
 L-proline purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
18.3
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
18.8
-
 L-proline purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C Azospirillum brasilense
18.8
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Colwellia psychrerythraea
80
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
80.1
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33312
-
 calculated from sequence Azospirillum brasilense
36182
-
 calculated from sequence Colwellia psychrerythraea
37000
-
 SDS-PAGE Azospirillum brasilense
40000
-
 SDS-PAGE Colwellia psychrerythraea
62000
-
 recombinant enzyme, gel filtration Azospirillum brasilense
78000
-
 gel filtration Azospirillum brasilense
81000
-
 gel filtration Colwellia psychrerythraea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-piperideine-2-carboxylate + NAD(P)H + H+ Azospirillum brasilense
-
 L-pipecolate + NAD(P)+
-
 r
1-pyrroline-2-carboxylate + NAD(P)H + H+ Azospirillum brasilense
-
 L-proline + NAD(P)+
-
 r
additional information Azospirillum brasilense trans-3-hydroxy-L-proline is converted to proline by the purified LhpH and LhpI proteins, i.e. trans-3-hydroxy-L-proline dehydratase and DELTA1-pyrroline-2-carboxylate reductase, at pH 7.0. LhpI has dual metabolic functions as a reductase for DELTA1-pyrroline-2-carboxylate and DELTA1-piperidine-2-carboxylate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense V5YW53
-
-
Colwellia psychrerythraea Q485R8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the organsim can grow on trans-3-hydroxy-L-proline and trans-4-hydroxy-L-proline as sole carbom sources, but also on D-proline and D-lysine Azospirillum brasilense
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000457
-
 substrates: L-pipecolate, NAD+, pH 10.5, 30°C Colwellia psychrerythraea
0.0005
-
 purified recombinant His-tagged enzyme, substrates L-pipecolate and NAD+, pH 6.5, 30°C Azospirillum brasilense
0.00435
-
 substrates: L-pipecolate, NADP+, pH 10.5, 30°C Colwellia psychrerythraea
0.0044
-
 purified recombinant His-tagged enzyme, substrates L-pipecolate and NADP+, pH 6.5, 30°C Azospirillum brasilense
0.0045
-
 purified recombinant His-tagged enzyme, substrates L-proline and NAD+, pH 6.5, 30°C Azospirillum brasilense
0.00451
-
 substrates: L-proline, NAD+, pH 10.5, 30°C Colwellia psychrerythraea
0.0464
-
 substrates: DELTA1-piperidine-2-carboxylate, NADH, pH 6.5, 30°C Colwellia psychrerythraea
0.174
-
 substrates: (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate, NADPH, pH 6.5, 30°C Colwellia psychrerythraea
0.291
-
 substrates: DELTA1-piperidine-2-carboxylate, NADPH, pH 6.5, 30°C Colwellia psychrerythraea
0.63
-
 purified recombinant His-tagged enzyme, substrates L-proline and NADP+, pH 6.5, 30°C Azospirillum brasilense
0.63
-
 substrates: L-proline, NADP+, pH 10.5, 30°C Colwellia psychrerythraea
2.72
-
 substrates: L-pipecolate, NAD+, pH 10.5, 30°C Azospirillum brasilense
2.92
-
 substrates: L-pipecolate, NADP+, pH 10.5, 30°C Azospirillum brasilense
4.76
-
 substrates: DELTA1-pyrroline-2-carboxylate, NADH, pH 6.5, 30°C Colwellia psychrerythraea
5.18
-
 substrates: L-proline, NADP+, pH 10.5, 30°C Azospirillum brasilense
5.5
-
 substrates: L-proline, NAD+, pH 10.5, 30°C Azospirillum brasilense
12
-
 purified recombinant His-tagged enzyme, substrates 1-pyrroline-(4R)-hydroxy-2-carboxylate and NADH, pH 6.5, 30°C Azospirillum brasilense
12
-
 substrates: DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate, NADPH, pH 6.5, 30°C Azospirillum brasilense
30.2
-
 substrates: DELTA1-pyrroline-2-carboxylate, NADPH, pH 6.5, 30°C Colwellia psychrerythraea
177
-
 purified recombinant His-tagged enzyme, substrates 1-piperideine-2-carboxylate and NADH, pH 6.5, 30°C Azospirillum brasilense
177
-
 substrates: DELTA1-piperidine-2-carboxylate, NADH, pH 6.5, 30°C Azospirillum brasilense
220
-
 purified recombinant His-tagged enzyme, substrates 1-piperideine-2-carboxylate and NADPH, pH 6.5, 30°C Azospirillum brasilense
220
-
 substrates: DELTA1-piperidine-2-carboxylate, NADPH, pH 6.5, 30°C Azospirillum brasilense
584
-
 purified recombinant His-tagged enzyme, substrates 1-pyrroline-2-carboxylate and NADPH, pH 6.5, 30°C Azospirillum brasilense
584
-
 substrates: DELTA1-pyrroline-2-carboxylate, NADPH, pH 6.5, 30°C Azospirillum brasilense
600
-
 purified recombinant His-tagged enzyme, substrates 1-pyrroline-2-carboxylate and NADH, pH 6.5, 30°C Azospirillum brasilense
600
-
 substrates: DELTA1-pyrroline-2-carboxylate, NADH, pH 6.5, 30°C Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate + NADPH + H+
-
 Colwellia psychrerythraea (4R)-4-hydroxy-L-proline + NADP+
-
 r
1-piperideine-2-carboxylate + NAD(P)H + H+
-
 Azospirillum brasilense L-pipecolate + NAD(P)+
-
 r
1-pyrroline-(4R)-hydroxy-2-carboxylate + NADPH + H+
-
 Azospirillum brasilense (4R)-hydroxy-L-proline + NADP+
-
 r
1-pyrroline-2-carboxylate + NAD(P)H + H+
-
 Azospirillum brasilense L-proline + NAD(P)+
-
 r
cis-3-hydroxy-L-proline + NADP+ low activity Azospirillum brasilense ? + NADPH + H+
-
 r
cis-4-hydroxy-L-proline + NADP+ low activity Azospirillum brasilense ? + NADPH + H+
-
 r
DELTA1-piperidine-2-carboxylate + NADH + H+
-
 Azospirillum brasilense L-pipecolate + NAD+
-
 r
DELTA1-piperidine-2-carboxylate + NADH + H+
-
 Colwellia psychrerythraea L-pipecolate + NAD+
-
 r
DELTA1-piperidine-2-carboxylate + NADPH + H+
-
 Azospirillum brasilense L-pipecolate + NADP+
-
 r
DELTA1-piperidine-2-carboxylate + NADPH + H+
-
 Colwellia psychrerythraea L-pipecolate + NADP+
-
 r
DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate + NADPH + H+
-
 Azospirillum brasilense (4R)-hydroxy-L-proline + NADP+
-
 r
DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 Azospirillum brasilense L-proline + NAD+
-
 r
DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 Colwellia psychrerythraea L-proline + NAD+
-
 r
DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 Azospirillum brasilense L-proline + NADP+
-
 r
DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 Colwellia psychrerythraea L-proline + NADP+
-
 r
L-pipecolate + NAD(P)+
-
 Azospirillum brasilense 1-piperideine-2-carboxylate + NAD(P)H + H+
-
 r
L-pipecolate + NAD+
-
 Azospirillum brasilense DELTA1-piperidine-2-carboxylate + NADH + H+
-
 r
L-pipecolate + NAD+
-
 Colwellia psychrerythraea DELTA1-piperidine-2-carboxylate + NADH + H+
-
 r
L-pipecolate + NADP+
-
 Azospirillum brasilense DELTA1-piperidine-2-carboxylate + NADPH + H+
-
 r
L-pipecolate + NADP+
-
 Colwellia psychrerythraea DELTA1-piperidine-2-carboxylate + NADPH + H+
-
 r
L-proline + NAD(P)+
-
 Azospirillum brasilense 1-pyrroline-2-carboxylate + NAD(P)H + H+
-
 r
L-proline + NAD+
-
 Azospirillum brasilense DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 r
L-proline + NAD+
-
 Colwellia psychrerythraea DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 r
L-proline + NADP+
-
 Azospirillum brasilense DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 r
L-proline + NADP+
-
 Colwellia psychrerythraea DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 r
additional information trans-3-hydroxy-L-proline is converted to proline by the purified LhpH and LhpI proteins, i.e. trans-3-hydroxy-L-proline dehydratase and DELTA1-pyrroline-2-carboxylate reductase, at pH 7.0. LhpI has dual metabolic functions as a reductase for DELTA1-pyrroline-2-carboxylate and DELTA1-piperidine-2-carboxylate Azospirillum brasilense ?
-
 ?
additional information no activity with trans-4-hydroxy-D-proline, D-proline, trans-3-hydroxy-L-proline, cis-4-hydroxy-D-proline, and cis-3-hydroxy-D-proline, poor activity with trans-4-hydroxy-L-proline Azospirillum brasilense ?
-
 ?
trans-4-hydroxy-L-proline + NADP+ low activity Azospirillum brasilense ? + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Colwellia psychrerythraea
dimer 2 * 37000, SDS-PAGE Azospirillum brasilense
dimer 2 * 33312, calculated from sequence Azospirillum brasilense
dimer 2 * 36182, calculated from sequence Colwellia psychrerythraea
homodimer 2 * 33313, sequence calculation, 2 * 37000, recombinant enzyme, SDS-PAGE Azospirillum brasilense

Synonyms

Synonyms Comment Organism
CphpI
-
 Colwellia psychrerythraea
DELTA1-Pyr2C reductase
-
 Azospirillum brasilense
DELTA1-Pyr2C reductase
-
 Colwellia psychrerythraea
DELTA1-pyrroline-2-carboxylate reductase
-
 Azospirillum brasilense
DELTA1-pyrroline-2-carboxylate reductase
-
 Colwellia psychrerythraea
lhpI
-
 Azospirillum brasilense
NAD(P)H-dependent DELTA1-pyrroline-2-carboxylate reductase
-
 Azospirillum brasilense
Pyr2C reductase
-
 Azospirillum brasilense
Pyr2C/Pip2C reductase
-
 Azospirillum brasilense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Azospirillum brasilense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0069
-
 L-proline purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C Azospirillum brasilense
0.0069
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Colwellia psychrerythraea
0.02
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
0.0213
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
0.088
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
1.02
-
 (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
1.09
-
 L-proline purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
1.09
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
1.38
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
2.5
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
3.7
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
3.92
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
4.23
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
10.35
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
10.35
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
10.35
-
 DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
124.5
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
161.5
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
161.5
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
165.8
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
166
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
523
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
523.3
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
615
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
615
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 reduction reaction Azospirillum brasilense
10.5
-
 oxidation reaction Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme shows dual specificity for NADPH and NADH Azospirillum brasilense
NADH
-
 Azospirillum brasilense
NADPH
-
 Azospirillum brasilense

Expression

Organism Comment Expression
Azospirillum brasilense trans-3-hydroxy-L-proline dehydratase and NAD(P)H-dependent DELTA1-pyrroline-2-carboxylate reductase activity is induced by trans-3-hydroxy-L-proline, D-proline, and D-lysine, but not by trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline up
Azospirillum brasilense induced by trans-3-hydroxy-L-proline (and D-proline and D-lysine) but not trans-4-hydroxy-L-proline up

General Information

General Information Comment Organism
evolution enzyme Pyr2C reductase is a member of the ornithine cyclodeaminase/micro-crystallin superfamily, different from known dpkA protein, and there are several significant differences in the enzymatic properties between Azospirillum brasilense and another bacteria: substrate and coenzyme specificities, phylogenetic analysis Azospirillum brasilense
malfunction disruption of LhpI gene from Azospirillum brasilense leads to loss of growth on trans-3-hydroxy-L-proline (T3LHyp), D-proline and D-lysine Azospirillum brasilense
malfunction disruption of LhpI gene from Azospirillum brasilense leads to loss of growth on trans-3-hydroxy-L-proline, D-proline and D-lysine, indicating that this gene has dual metabolic functions as a reductase for Pyr2C and DELTA1-piperidine-2-carboxylate in these pathways, and that the T3LHyp pathway is not linked to trans-4-hydroxy-L-proline and L-proline metabolism Azospirillum brasilense
metabolism the enzyme is involved in the trans-3-hydroxy-L-proline pathway and the metabolic networks with D-lysine and D-proline, overview. The trans-3-hydroxy-L-proline pathway is not linked to trans-4-hydroxy-L-proline and L-proline metabolism Azospirillum brasilense
physiological function enzyme AbLhpI is a bifunctional NAD(P)H-dependent Delta1-pyrroline-2-carboxylate/Delta1-piperideine-2-carboxylate reductase (Pyr2C/Pip2C reductase) Azospirillum brasilense

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000027
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
0.00027
-
 L-pipecolate purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
0.00037
-
 L-proline purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C Azospirillum brasilense
0.00037
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Colwellia psychrerythraea
0.0418
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
0.0595
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Colwellia psychrerythraea
0.06
-
 L-proline purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C Azospirillum brasilense
0.124
-
 (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
0.19
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
0.25
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
0.38
-
 L-pipecolate pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
1.06
-
 L-proline pH 10.5, 30°C, coenzyme: NAD+ Azospirillum brasilense
1.08
-
 L-proline pH 10.5, 30°C, coenzyme: NADP+ Azospirillum brasilense
21.1
-
 1-pyrroline-(4R)-hydroxy-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
21.2
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Colwellia psychrerythraea
21.2
-
 DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
225
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Colwellia psychrerythraea
269
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
270
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
350
-
 1-piperideine-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
351.7
-
 DELTA1-piperidine-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense
735
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C Azospirillum brasilense
735
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADH Azospirillum brasilense
833
-
 1-pyrroline-2-carboxylate purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C Azospirillum brasilense
835
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, coenzyme: NADPH Azospirillum brasilense