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Literature summary for 1.4.99.B4 extracted from

  • Watanabe, S.; Morimoto, D.; Fukumori, F.; Watanabe, Y.
    Characterization of cis-4-hydroxy-D-proline dehydrogenase from Sinorhizobium meliloti (2018), Biosci. Biotechnol. Biochem., 82, 110-113 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0602
-
cis-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
0.188
-
cis-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
0.2
-
trans-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
0.401
-
D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
0.473
-
trans-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
0.942
-
D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
2 3 D-methionine pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
16.5
-
D-pipecolate pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-4-hydroxy-D-proline + acceptor Sinorhizobium meliloti
-
(4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti Q92WS2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0224
-
substrate: D-methionine, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
0.759
-
substrate: D-pipecolate, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
1.32
-
substrate: D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
1.38
-
substrate: trans-4-hydroxy-D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
1.4
-
substrate: cis-4-hydroxy-D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
1.84
-
substrate: trans-4-hydroxy-D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
2
-
substrate: D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
3.92
-
substrate: cis-4-hydroxy-D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-4-hydroxy-D-proline + acceptor
-
Sinorhizobium meliloti (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate + reduced acceptor
-
?
D-methionine + acceptor + acceptor
-
Sinorhizobium meliloti ?
-
?
D-pipecolate + acceptor + acceptor
-
Sinorhizobium meliloti ?
-
?
D-proline + acceptor + acceptor
-
Sinorhizobium meliloti ?
-
?
trans-4-hydroxy-D-proline + acceptor
-
Sinorhizobium meliloti ?
-
?

Synonyms

Synonyms Comment Organism
SM_b20267
-
Sinorhizobium meliloti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.057
-
D-methionine pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
1.02
-
cis-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
1.07
-
trans-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
1.12
-
D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
1.68
-
trans-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
1.92
-
D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
2.08
-
cis-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
2.9
-
D-pipecolate pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 9.75
-
Sinorhizobium meliloti

Cofactor

Cofactor Comment Organism Structure
FAD FAD-containing enzyme Sinorhizobium meliloti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.18
-
D-pipecolate pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
2.03
-
D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
2.47
-
D-methionine pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
2.82
-
D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
3.65
-
trans-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
5.37
-
trans-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti
11.06
-
cis-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet Sinorhizobium meliloti
16.9
-
cis-4-hydroxy-D-proline pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium Sinorhizobium meliloti