BRENDA - Enzyme Database show
show all sequences of 1.4.99.B3

Purification and characterization of an L-amino acid deaminase used to prepare unnatural amino acids

Panataleone, D.P.; Geller, A.M.; Taylor, P.P.; J. Mol. Catal. B 11, 795-803 (2001)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Cosenzaea myxofaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.28
-
L-phenylalanine
pH 7.5, 30°C
Cosenzaea myxofaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51000
-
x * 51000, SDS-PAGE
Cosenzaea myxofaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cosenzaea myxofaciens
A0A158RFS7
-
-
Cosenzaea myxofaciens 4482
A0A158RFS7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-cysteine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b
71.5% of the activiy with L-phenylalanine
-
-
?
L-cysteine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens 4482
2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b
71.5% of the activiy with L-phenylalanine
-
-
?
L-isoleucine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b
27.8% of the activiy with L-phenylalanine
-
-
?
L-isoleucine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens 4482
3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b
27.8% of the activiy with L-phenylalanine
-
-
?
L-leucine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b
111% of the activiy with L-phenylalanine
-
-
?
L-leucine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens 4482
2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b
111% of the activiy with L-phenylalanine
-
-
?
L-methionine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b
102% of the activiy with L-phenylalanine
-
-
?
L-methionine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens 4482
4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b
102% of the activiy with L-phenylalanine
-
-
?
L-phenylalanine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
phenylpyruvate + NH3 + 2 reduced cytochrome b
-
-
-
?
L-tryptophan + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + 2 reduced cytochrome b
90.6% of the activiy with L-phenylalanine
-
-
?
L-tyrosine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
4-hydroxyphenylpyruvate + NH3 + 2 reduced cytochrome b
38.5% of the activiy with L-phenylalanine
-
-
?
additional information
aliphatic, aromatic or sulfur-containing amino acids are preferred. The reaction has an absolute requirement for O2, releases NH3 and does not produce H2O2
740873
Cosenzaea myxofaciens
?
-
-
-
-
additional information
aliphatic, aromatic or sulfur-containing amino acids are preferred. The reaction has an absolute requirement for O2, releases NH3 and does not produce H2O2
740873
Cosenzaea myxofaciens 4482
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 51000, SDS-PAGE
Cosenzaea myxofaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Cosenzaea myxofaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Cosenzaea myxofaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.28
-
L-phenylalanine
pH 7.5, 30°C
Cosenzaea myxofaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51000
-
x * 51000, SDS-PAGE
Cosenzaea myxofaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-cysteine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b
71.5% of the activiy with L-phenylalanine
-
-
?
L-cysteine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens 4482
2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b
71.5% of the activiy with L-phenylalanine
-
-
?
L-isoleucine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b
27.8% of the activiy with L-phenylalanine
-
-
?
L-isoleucine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens 4482
3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b
27.8% of the activiy with L-phenylalanine
-
-
?
L-leucine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b
111% of the activiy with L-phenylalanine
-
-
?
L-leucine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens 4482
2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b
111% of the activiy with L-phenylalanine
-
-
?
L-methionine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b
102% of the activiy with L-phenylalanine
-
-
?
L-methionine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens 4482
4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b
102% of the activiy with L-phenylalanine
-
-
?
L-phenylalanine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
phenylpyruvate + NH3 + 2 reduced cytochrome b
-
-
-
?
L-tryptophan + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + 2 reduced cytochrome b
90.6% of the activiy with L-phenylalanine
-
-
?
L-tyrosine + H2O + 2 cytochrome b
-
740873
Cosenzaea myxofaciens
4-hydroxyphenylpyruvate + NH3 + 2 reduced cytochrome b
38.5% of the activiy with L-phenylalanine
-
-
?
additional information
aliphatic, aromatic or sulfur-containing amino acids are preferred. The reaction has an absolute requirement for O2, releases NH3 and does not produce H2O2
740873
Cosenzaea myxofaciens
?
-
-
-
-
additional information
aliphatic, aromatic or sulfur-containing amino acids are preferred. The reaction has an absolute requirement for O2, releases NH3 and does not produce H2O2
740873
Cosenzaea myxofaciens 4482
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 51000, SDS-PAGE
Cosenzaea myxofaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Cosenzaea myxofaciens
Other publictions for EC 1.4.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740746
Motta
Structure-function relationshi ...
Cosenzaea myxofaciens
J. Biol. Chem.
291
10457-75
2016
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-
1
1
1
1
1
1
2
-
2
-
-
3
-
-
-
-
-
-
1
2
11
1
1
1
-
1
1
1
-
1
-
-
-
-
-
1
1
1
1
1
-
1
-
1
2
-
2
-
-
-
-
-
-
-
1
2
11
1
1
1
-
1
1
1
-
-
-
-
-
-
-
-
740873
Panataleone
-
Purification and characterizat ...
Cosenzaea myxofaciens, Cosenzaea myxofaciens 4482
J. Mol. Catal. B
11
795-803
2001
-
-
1
-
-
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
13
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
13
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391786
Pelmont
L-Amino acid oxidases of Prote ...
Proteus mirabilis
Biochimie
54
1359-1374
1972
-
-
-
-
-
-
-
-
1
-
-
-
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1
-
-
-
-
-
-
-
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9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
1
-
-
-
-
-
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