Literature summary for 1.4.99.6 extracted from

Ouedraogo, D.; Souffrant, M.; Vasquez, S.; Hamelberg, D.; Gadda, G.
Importance of loop L1 dynamics for substrate capture and catalysis in Pseudomonas aeruginosa D-arginine dehydrogenase (2017), Biochemistry, 56, 2477-2487.

Search for more literature for 1.4.99.6

Cloned(Commentary)

Cloned (Comment)	Organism
-	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
A46G	mutation in loop L1. Probability of being in open conformation is higher than that of wild-type, yielding an increased level of solvent exposure of the active site. The flavin fluorescence intensity is about 2fold higher than in wild-type. Loop L1 is important for substrate capture and catalysis	Pseudomonas aeruginosa
S45A	mutation in loop L1. Probability of being in open conformation is higher than that of wild-type, yielding an increased level of solvent exposure of the active site. The flavin fluorescence intensity is about 2fold higher than in wild-type. Loop L1 is important for substrate capture and catalysis	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HXE3	-	-
Pseudomonas aeruginosa DSM 22644	Q9HXE3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-arginine + phenazine methosulfate + H2O	-	Pseudomonas aeruginosa	5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate	overall reaction	?
D-arginine + phenazine methosulfate + H2O	-	Pseudomonas aeruginosa DSM 22644	5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate	overall reaction	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.4	-	D-arginine	mutant S45A, pH 5.5, 25°C	Pseudomonas aeruginosa
23	-	D-arginine	mutant A46G, pH 6.5, 25°C	Pseudomonas aeruginosa
210	-	D-arginine	mutant A46G, pH 10.0, 25°C	Pseudomonas aeruginosa
290	-	D-arginine	mutant S45A, pH 10.0, 25°C	Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.014	-	D-arginine	mutant S45A, pH 5.5, 25°C	Pseudomonas aeruginosa
0.59	-	D-arginine	mutant A46G, pH 6.5, 25°C	Pseudomonas aeruginosa
270	-	D-arginine	mutant S45A, pH 10.0, 25°C	Pseudomonas aeruginosa
340	-	D-arginine	mutant A46G, pH 10.0, 25°C	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)