Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.99.6 extracted from

  • Gannavaram, S.; Sirin, S.; Sherman, W.; Gadda, G.
    Mechanistic and computational studies of the reductive half-reaction of tyrosine to phenylalanine active site variants of D-arginine dehydrogenase (2014), Biochemistry, 53, 6574-6583.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
Y249F steady-state kinetic parameters similar to those of the wild-type enzyme. Rate constants for flavin reduction (kred) with D-leucine are 3fold smaller than the wild-type value with similar pKa values for an unprotonated group of about 10.0. Cleavage of the substrate NH and CH bonds in the enzyme variant occurs in synchronous fashion, which can be reconciled with a hydride transfer mechanism Pseudomonas aeruginosa
Y53F steady-state kinetic parameters similar to those of the wild-type enzyme. Rate constants for flavin reduction (kred) with D-leucineare 3fold smaller than the wild-type value with similar pKa values for an unprotonated group of about 10.0. Cleavage of the substrate NH and CH bonds in the enzyme variant occurs in synchronous fashion, which can be reconciled with a hydride transfer mechanism Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
D-arginine mutant Y249F, pH 8.7, 25°C Pseudomonas aeruginosa
0.36
-
D-arginine mutant Y53F, pH 8.7, 25°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HXE3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arginine + phenazine methosulfate
-
Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate overall reaction ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
204
-
D-arginine mutant Y249F, pH 8.7, 25°C Pseudomonas aeruginosa
420
-
D-arginine mutant Y53F, pH 8.7, 25°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1200
-
D-arginine mutant Y53F, pH 8.7, 25°C Pseudomonas aeruginosa
3400
-
D-arginine mutant Y249F, pH 8.7, 25°C Pseudomonas aeruginosa