Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
Y249F | steady-state kinetic parameters similar to those of the wild-type enzyme. Rate constants for flavin reduction (kred) with D-leucine are 3fold smaller than the wild-type value with similar pKa values for an unprotonated group of about 10.0. Cleavage of the substrate NH and CH bonds in the enzyme variant occurs in synchronous fashion, which can be reconciled with a hydride transfer mechanism | Pseudomonas aeruginosa |
Y53F | steady-state kinetic parameters similar to those of the wild-type enzyme. Rate constants for flavin reduction (kred) with D-leucineare 3fold smaller than the wild-type value with similar pKa values for an unprotonated group of about 10.0. Cleavage of the substrate NH and CH bonds in the enzyme variant occurs in synchronous fashion, which can be reconciled with a hydride transfer mechanism | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-arginine | mutant Y249F, pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.36 | - |
D-arginine | mutant Y53F, pH 8.7, 25°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HXE3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arginine + phenazine methosulfate | - |
Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate | overall reaction | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
204 | - |
D-arginine | mutant Y249F, pH 8.7, 25°C | Pseudomonas aeruginosa | |
420 | - |
D-arginine | mutant Y53F, pH 8.7, 25°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1200 | - |
D-arginine | mutant Y53F, pH 8.7, 25°C | Pseudomonas aeruginosa | |
3400 | - |
D-arginine | mutant Y249F, pH 8.7, 25°C | Pseudomonas aeruginosa |