BRENDA - Enzyme Database show
show all sequences of 1.4.99.6

Heterologous expression of the Bacillus subtilis (natto) alanine dehydrogenase in Escherichia coli and Lactococcus lactis

Ye, W.; Huo, G.; Chen, J.; Liu, F.; Yin, J.; Yang, L.; Ma, X.; Microbiol. Res. 165, 268-275 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinantly expressed in Escherichia coli
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Inhibitors
Inhibitors
Commentary
Organism
Structure
Benzoic acid
0.1 mM, 98% inhibition
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
iodoacetamide
0.1 mM, 65.8% inhibition
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
iodoacetate
0.1 mM, 61.8% inhibition
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
p-hydroxymercuribenzoate
0.1 mM, 78.6% inhibition
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0082
-
D-proline
Vmax: 12.3 micromol/min/mg
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
40.2
-
D-proline
Vmax: 25 micromol/min/mg
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
SDS-PAGE
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
A3KEZ1
-
-
Purification (Commentary)
Commentary
Organism
using a DEAE-Toyopearl column
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.42
-
using D-serine as a substrate
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.25
-
using D-phenylalanine as a substrate
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
2.29
-
using D-alanine as a substrate
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
6.22
-
using D-proline as a substrate
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-alanine + H2O + 2,6-dichlorophenolindophenol
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-proline + H2O + 2,6-dichlorophenolindophenol
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-proline + H2O + coenzyme Q1
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
-
-
-
?
D-serine + H2O + 2,6-dichlorophenolindophenol
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
optimal temperature and assay at
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
optimal pH and assay at
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinantly expressed in Escherichia coli
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Benzoic acid
0.1 mM, 98% inhibition
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
iodoacetamide
0.1 mM, 65.8% inhibition
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
iodoacetate
0.1 mM, 61.8% inhibition
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
p-hydroxymercuribenzoate
0.1 mM, 78.6% inhibition
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0082
-
D-proline
Vmax: 12.3 micromol/min/mg
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
40.2
-
D-proline
Vmax: 25 micromol/min/mg
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
SDS-PAGE
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Purification (Commentary) (protein specific)
Commentary
Organism
using a DEAE-Toyopearl column
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.42
-
using D-serine as a substrate
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.25
-
using D-phenylalanine as a substrate
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
2.29
-
using D-alanine as a substrate
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
6.22
-
using D-proline as a substrate
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-alanine + H2O + 2,6-dichlorophenolindophenol
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-proline + H2O + 2,6-dichlorophenolindophenol
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-proline + H2O + coenzyme Q1
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
-
-
-
?
D-serine + H2O + 2,6-dichlorophenolindophenol
-
705545
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
optimal temperature and assay at
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
optimal pH and assay at
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Other publictions for EC 1.4.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740089
Ouedraogo
Importance of loop L1 dynamics ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644
Biochemistry
56
2477-2487
2017
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4
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4
4
733215
Ball
Importance of glutamate 87 and ...
Pseudomonas aeruginosa
Arch. Biochem. Biophys.
568
56-63
2015
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2
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2
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2
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4
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5
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8
8
724092
Akita
Efficient synthesis of D-branc ...
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1 (NBRC 108682)
Appl. Microbiol. Biotechnol.
98
1135-1143
2014
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1
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1
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2
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1
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6
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1
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1
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1
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1
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1
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6
-
1
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1
1
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1
1
733354
Gannavaram
Mechanistic and computational ...
Pseudomonas aeruginosa
Biochemistry
53
6574-6583
2014
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1
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2
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2
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2
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1
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2
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2
2
724690
Oliver
Impact of D-amino acid dehydro ...
Pseudomonas aeruginosa
Can. J. Microbiol.
59
598-603
2013
-
-
1
-
-
-
-
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-
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5
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1
1
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-
726275
Bardaweel
E. coli histidine triad nucleo ...
Escherichia coli
PLoS ONE
6
e20897
2011
1
-
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-
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6
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1
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1
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1
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733330
Fu
Atomic-resolution structure of ...
Pseudomonas aeruginosa
Biochemistry
50
6292-6294
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
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734025
Yuan
Insights on the mechanism of a ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
133
18957-18965
2011
-
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1
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1
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748083
He
Regulation and characterizati ...
Pseudomonas aeruginosa
J. Bacteriol.
193
2107-2115
2011
-
-
1
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-
9
1
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1
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3
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1
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10
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1
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9
1
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1
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1
1
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9
1
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1
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1
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10
-
1
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9
1
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-
1
3
3
1
9
9
695533
Tanigawa
D-Amino acid dehydrogenase fro ...
Helicobacter pylori, Helicobacter pylori NCTC 11637
Amino Acids
38
247-255
2010
-
-
1
-
-
-
5
2
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1
2
2
-
2
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1
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8
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16
1
1
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1
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1
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1
1
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5
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2
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1
2
2
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1
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8
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16
1
1
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1
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733326
Fu
Conformational changes and sub ...
Pseudomonas aeruginosa
Biochemistry
49
8535-8545
2010
-
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1
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7
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2
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7
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7
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1
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7
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7
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7
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7
7
733327
Yuan
Steady-state kinetic mechanism ...
Pseudomonas aeruginosa
Biochemistry
49
9542-9550
2010
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2
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2
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2
2
705545
Ye
Heterologous expression of the ...
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
Microbiol. Res.
165
268-275
2009
-
-
1
-
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-
4
2
-
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1
-
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1
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1
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4
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5
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1
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1
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1
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1
1
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4
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2
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1
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1
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4
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5
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1
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1
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705583
Li
Regulation of the dauBAR Opero ...
Pseudomonas aeruginosa
Microbiology
156
60-71
2009
-
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1
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2
7
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2
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7
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1
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7
1
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1
2
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1
1
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2
2
7
-
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7
-
1
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7
1
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1
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1
7
7
706547
Li
Arginine racemization by coupl ...
Pseudomonas aeruginosa
Proc. Natl. Acad. Sci. USA
106
906-911
2009
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1
1
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394974
Tsukada
-
D-Amino acid dehydrogenase (Ps ...
Pseudomonas fluorescens
Methods Enzymol.
17B
623-624
1971
-
-
-
-
-
1
3
4
-
-
-
-
-
1
-
-
1
-
-
1
2
-
30
-
-
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1
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1
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1
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1
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3
-
4
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1
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1
2
-
30
-
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1
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-
394975
Tsukada
D-Amino acid dehydrogenases of ...
Pseudomonas fluorescens
J. Biol. Chem.
241
4522-4528
1966
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1
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