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Literature summary for 1.4.99.6 extracted from

  • Tanigawa, M.; Shinohara, T.; Saito, M.; Nishimura, K.; Hasegawa, Y.; Wakabayashi, S.; Ishizuka, M.; Nagata, Y.D
    D-Amino acid dehydrogenase from Helicobacter pylori NCTC 11637 (2010), Amino Acids, 38, 247-255.
    View publication on PubMed
Search for more literature for 1.4.99.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxybenzoic acid 21.4% residual activity at 0.1 mM Helicobacter pylori
benzoate 2% residual activity at 0.1 mM Helicobacter pylori
iodoacetamide 34.2% residual activity at 0.1 mM Helicobacter pylori
iodoacetate 38.2% residual activity at 0.1 mM Helicobacter pylori
additional information not inhibited by EDTA Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
 coenzyme Q1 in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
40.2
-
 D-Pro with Q1 as the electron acceptor, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Mg2+ and Ca+ Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, SDS-PAGE Helicobacter pylori
46040
-
 x * 46040, calculated from amino acid sequence Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Pro + H2O + coenzyme Q1 Helicobacter pylori D-Pro is the best substrate ?
-
 ?
D-Pro + H2O + coenzyme Q1 Helicobacter pylori NCTC 11637 D-Pro is the best substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori A3KEZ1
-
-
Helicobacter pylori NCTC 11637 A3KEZ1
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography Helicobacter pylori

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
 purified enzyme, with 20 mM D-Asp as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
0.05
-
 purified enzyme, with 20 mM D-Met as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
0.05
-
 purified enzyme, with 20 mM D-Val as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
0.06
-
 purified enzyme, with 20 mM D-Glu as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
0.12
-
 purified enzyme, with 20 mM L-Ala as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
0.22
-
 purified enzyme, with 20 mM L-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
0.46
-
 cell-free extract, with 20 mM D-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.25
-
 purified enzyme, with 20 mM D-Ser as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
2.29
-
 purified enzyme, with 20 mM D-Ala as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
6.22
-
 purified enzyme, with 20 mM D-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol 36.8% activity compared to D-Pro Helicobacter pylori pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol 36.8% activity compared to D-Pro Helicobacter pylori NCTC 11637 pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori 2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori NCTC 11637 2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol 1.0% activity compared to D-Pro Helicobacter pylori 2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol 1.0% activity compared to D-Pro Helicobacter pylori NCTC 11637 2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Met + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Met + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori NCTC 11637 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Phe + H2O + oxidized 2,6-dichlorophenolindophenol 19.8% activity compared to D-Pro Helicobacter pylori phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Pro + H2O + coenzyme Q1 D-Pro is the best substrate Helicobacter pylori ?
-
 ?
D-Pro + H2O + coenzyme Q1 D-Pro is the best substrate Helicobacter pylori NCTC 11637 ?
-
 ?
D-Pro + H2O + oxidized 2,6-dichlorophenolindophenol 100% activity Helicobacter pylori DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Ser + H2O + oxidized 2,6-dichlorophenolindophenol 6.7% activity compared to D-Pro Helicobacter pylori 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-Val + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
L-Ala + H2O + oxidized 2,6-dichlorophenolindophenol 1.9% activity compared to D-Pro Helicobacter pylori ?
-
 ?
L-Pro + H2O + oxidized 2,6-dichlorophenolindophenol 3.5% activity compared to D-Pro Helicobacter pylori ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Helicobacter pylori
? x * 46040, calculated from amino acid sequence Helicobacter pylori

Synonyms

Synonyms Comment Organism
D-amino acid dehydrogenase
-
 Helicobacter pylori
DAD
-
 Helicobacter pylori
DadA
-
 Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Helicobacter pylori