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Literature summary for 1.4.9.1 extracted from
- Biochemical and electrochemical characterization of quinohemoprotein amine dehydrogenase from Paracoccus denitrificans (1999), Biochemistry, 38, 6935-6942.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydrazine | - |
Paracoccus denitrificans |  |
| hydroxylamine | - |
Paracoccus denitrificans | |
| phenylhydrazine | - |
Paracoccus denitrificans | |
| Semicarbazide | - |
Paracoccus denitrificans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
phenazine ethosulfate | - |
Paracoccus denitrificans | |
| 0.1 | - |
ethylamine | - |
Paracoccus denitrificans | |
| 0.12 | - |
2,6-dichloroindophenol | - |
Paracoccus denitrificans | |
| 0.78 | - |
K3Fe(CN)6 | - |
Paracoccus denitrificans | |
| 1.3 | - |
methylamine | - |
Paracoccus denitrificans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Paracoccus denitrificans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36500 | - |
alpha,beta, 1 * 59500 + 1 * 36500, SDS-PAGE | Paracoccus denitrificans |
| 59500 | - |
alpha,beta, 1 * 59500 + 1 * 36500, SDS-PAGE | Paracoccus denitrificans |
| 100000 | - |
gel filtration | Paracoccus denitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
- |
- |
| Paracoccus denitrificans IFO 12442 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzylamine + acceptor + H2O | - |
Paracoccus denitrificans | benzaldehyde + NH3 + reduced acceptor | - |
? | |
| benzylamine + acceptor + H2O | - |
Paracoccus denitrificans IFO 12442 | benzaldehyde + NH3 + reduced acceptor | - |
? | |
| butylamine + acceptor + H2O | - |
Paracoccus denitrificans | butanal + NH3 + reduced acceptor | - |
? | |
| butylamine + acceptor + H2O | - |
Paracoccus denitrificans IFO 12442 | butanal + NH3 + reduced acceptor | - |
? | |
| ethylamine + acceptor + H2O | - |
Paracoccus denitrificans | acetaldehyde + NH3 + reduced acceptor | - |
? | |
| methylamine + acceptor + H2O | - |
Paracoccus denitrificans | methanal + NH3 + reduced acceptor | - |
? | |
| methylamine + acceptor + H2O | - |
Paracoccus denitrificans IFO 12442 | methanal + NH3 + reduced acceptor | - |
? | |
| methylamine + H2O + 2,6-dichloroindophenol | - |
Paracoccus denitrificans | formaldehyde + NH3 + reduced 2,6-dichloroindophenol | - |
r | |
| methylamine + H2O + 2,6-dichloroindophenol + phenazine ethosulfate | - |
Paracoccus denitrificans | formaldehyde + NH3 + reduced phenazine ethosulfate + ? | - |
r | |
| methylamine + H2O + K3Fe(CN)6 | - |
Paracoccus denitrificans | formaldehyde + NH3 + reduced K3Fe(CN)6 | - |
r | |
| phenylethylamine + acceptor + H2O | - |
Paracoccus denitrificans | phenylacetaldehyde + NH3 + reduced acceptor | - |
? | |
| propylamine + acceptor + H2O | - |
Paracoccus denitrificans | propionaldehyde + NH3 + reduced acceptor | - |
? | |
| propylamine + acceptor + H2O | - |
Paracoccus denitrificans IFO 12442 | propionaldehyde + NH3 + reduced acceptor | - |
? | |
| RCH2NH2 + acceptor + H2O | acceptor potassium ferricyanide, phenazine ethosulfate, 2,6-dichlorophenolindophenol | Paracoccus denitrificans | RCHO + NH3 + reduced acceptor | - |
? | |
| RCH2NH2 + acceptor + H2O | acceptor potassium ferricyanide, phenazine ethosulfate, 2,6-dichlorophenolindophenol | Paracoccus denitrificans IFO 12442 | RCHO + NH3 + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha,beta, 1 * 59500 + 1 * 36500, SDS-PAGE | Paracoccus denitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| QH-AmDH | different from MADH | Paracoccus denitrificans |
| quinohemoprotein amine dehydrogenase | different from MADH | Paracoccus denitrificans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
2,6-dichloroindophenol | - |
Paracoccus denitrificans | |
| 7.1 | - |
phenazine ethosulfate | - |
Paracoccus denitrificans | |
| 13 | - |
ethylamine | - |
Paracoccus denitrificans | |
| 14 | - |
methylamine | - |
Paracoccus denitrificans | |
| 15 | - |
K3Fe(CN)6 | - |
Paracoccus denitrificans | |
| 22 | - |
benzylamine | - |
Paracoccus denitrificans | |
| 22 | - |
Butylamine | - |
Paracoccus denitrificans | |
| 26 | - |
phenethylamine | - |
Paracoccus denitrificans | |
| 27 | - |
Propylamine | - |
Paracoccus denitrificans | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme c | contains 1 mol heme c per mol enzyme in the alpha subunit | Paracoccus denitrificans | |
| quinoid cofactor | alpha subunit contains unknown quinoid cofactor | Paracoccus denitrificans |
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