Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM105 cells | Leptolyngbya boryana |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 22-23% (w/v) PEG 3350, 170 mM sodium acetate trihydrate pH 7.0, 85 mM Tris-HCl pH 8.2, 15% (v/v) glycerol | Leptolyngbya boryana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
gel filtration | Leptolyngbya boryana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + reduced ferredoxin | Leptolyngbya boryana | - |
L-glutamate + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptolyngbya boryana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ferredoxin-immobilized Sepharose column chromatography | Leptolyngbya boryana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + reduced ferredoxin | - |
Leptolyngbya boryana | L-glutamate + oxidized ferredoxin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fd-GOGAT | - |
Leptolyngbya boryana |
ferredoxin-dependent glutamate synthase | - |
Leptolyngbya boryana |
glutamine 2-oxoglutarate amidotransferase | - |
Leptolyngbya boryana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Leptolyngbya boryana | |
[3Fe-4S]-center | - |
Leptolyngbya boryana |