Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no stimulation of activity by FAD, pyridoxalphosphate, NAD+, NADP+ | Phormia regina |
General Stability | Organism |
---|---|
dialysis causes inactivation, activity cannot be restored by addition of pyridoxal phosphate, FAD, NAD+, NADP+ alone or in combination with divalent cations | Phormia regina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Phormia regina | |
Co2+ | - |
Phormia regina | |
cysteine | - |
Phormia regina | |
Dibenzylamine | - |
Phormia regina | |
glutathione | no effect | Phormia regina | |
hydrazine | - |
Phormia regina | |
hydroxylamine | - |
Phormia regina | |
KCN | - |
Phormia regina | |
additional information | not: 8-hydroxyquinoline | Phormia regina | |
Quinacrine | - |
Phormia regina | |
Semicarbazide | - |
Phormia regina | |
Sodium azide | - |
Phormia regina | |
Sodium bisulfite | - |
Phormia regina | |
sodium-diethyldithiocarbamate | - |
Phormia regina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
ethanolamine | - |
Phormia regina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Phormia regina | 5737 | - |
additional information | no activity in mitochondria | Phormia regina | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates the dialyzed enzyme, regeneration of 50% of the initial activity | Phormia regina | |
Ni2+ | slight stimulation | Phormia regina | |
Zn2+ | activates the dialyzed enzyme, regeneration of 50% of initial activity | Phormia regina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phormia regina | - |
blowfly | - |
Purification (Comment) | Organism |
---|---|
- |
Phormia regina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | low activity | Phormia regina | - |
larva | - |
Phormia regina | - |
pupa | low activity | Phormia regina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00026 | - |
- |
Phormia regina |
Storage Stability | Organism |
---|---|
more unstable, cannot be stored | Phormia regina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine + H2O + O2 | under aerobic conditions | Phormia regina | glycolaldehyde + NH3 + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Phormia regina |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | 48 | half-maximal activities at 26°C and 48°C | Phormia regina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Phormia regina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | pH 7.0: about 50% of activity maximum, pH 8.2: 37% of activity maximum | Phormia regina |