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Literature summary for 1.4.3.8 extracted from
- Metabolism of ethanolamine, an ethanoloxidase (1964), J. Biol. Chem., 239, 2189-2193.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no stimulation of activity by FAD, pyridoxalphosphate, NAD+, NADP+, FMN or tetrazolium dyes | Pseudomonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Pseudomonas sp. |  |
| cysteine | - |
Pseudomonas sp. | |
| glutathione | - |
Pseudomonas sp. | |
| hydrazine | - |
Pseudomonas sp. | |
| hydroxylamine | - |
Pseudomonas sp. | |
| Iproniazide | - |
Pseudomonas sp. | |
| isoniazide | low inhibition | Pseudomonas sp. | |
| Quinacrine | - |
Pseudomonas sp. | |
| Semicarbazide | - |
Pseudomonas sp. | |
| Sodium bisulfite | - |
Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
ethanolamine | - |
Pseudomonas sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanolamine + H2O + O2 | Pseudomonas sp. | aerobic catabolism of ethanolamine | glycolaldehyde + NH3 + H2O2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
Bacterium ATCC 13796, Arthrobacter sp. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | enzyme more active in larvae than in adults | Pseudomonas sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0621 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-amino-2-propanol + H2O + O2 | 18% of the activity with ethanolamine | Pseudomonas sp. | 2-hydroxy-propionic aldehyde + NH3 + H2O2 | - |
? | |
| 3-amino-1-propanol + H2O + O2 | 56% of the activity with ethanolamine | Pseudomonas sp. | 3-hydroxy-propionic aldehyde + NH3 + H2O2 | - |
? | |
| ethanolamine + H2O + O2 | under aerobic conditions | Pseudomonas sp. | glycolaldehyde + NH3 + H2O2 | - |
ir | |
| ethanolamine + H2O + O2 | aerobic catabolism of ethanolamine | Pseudomonas sp. | glycolaldehyde + NH3 + H2O2 | - |
ir | |
| additional information | no activity with: ethylamine, n-propylamine, putrescine, choline and phosphoethanolamine | Pseudomonas sp. | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.8 | half-maximal activities at pH 7 and pH 9.8 | Pseudomonas sp. |
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