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Literature summary for 1.4.3.5 extracted from

  • Wada, H.; Snell, E.E.
    The enzymatic oxidation of pyridoxine and pyridoxamine phosphates (1961), J. Biol. Chem., 236, 2089-2095.
    View publication on PubMed
Search for more literature for 1.4.3.5

Activating Compound

Activating Compound Comment Organism Structure
2-aminobutanoate activation Oryctolagus cuniculus
alanine activation Oryctolagus cuniculus
cysteine activation Oryctolagus cuniculus
Tris activation Oryctolagus cuniculus
valine activation Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.1 mM, 90-95% inhibition Oryctolagus cuniculus
4-deoxypyridoxine 5'-phosphate 0.1 mM, 42% inhibition Oryctolagus cuniculus
4-Pyridoxic acid phosphate 0.1 mM, 33.5% inhibition Oryctolagus cuniculus
Cu2+ 1 mM, 90-95% inhibition Oryctolagus cuniculus
Hg2+ 1 mM, 90-95% inhibition Oryctolagus cuniculus
pyridoxal 5'-phosphate
-
 Oryctolagus cuniculus
pyridoxaloxime 5'-phosphate 0.2 mM, 80% inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000031
-
 FMN
-
 Oryctolagus cuniculus
0.0031
-
 pyridoxine 5'-phosphate
-
 Oryctolagus cuniculus
0.14
-
 pyridoxamine 5'-phosphate
-
 Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.432
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10 pyridoxine 5'-phosphate, pyridoxamine 5'-phosphate Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
FAD about 0.1% as active as FMN Oryctolagus cuniculus
FMN flavoprotein Oryctolagus cuniculus