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Literature summary for 1.4.3.4 extracted from

  • Geha, R.M.; Chen, K.; Wouters, J.; Ooms, F.; Shih, J.C.
    Analysis of conserved active site residues in monoamine oxidase A and B and their three-dimensional molecular modeling (2002), J. Biol. Chem., 277, 17209-17216.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf21 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D123A 2.4fold increase in Km-value for phenylethylamine, 3.3fold increase in IC50-value for clorgyline, 3.3fold increase in IC50-value for deprenyl Homo sapiens
D132A 2.2fold increase in Km-value for serotonin,1.8fold increase in IC50-value for clorgyline, 3.9fold increase in IC50-value for deprenyl Homo sapiens
K296A no activity with phenylethylamine Homo sapiens
K305A no detectable activity with serotonin Homo sapiens
W388A no activity with phenylethylamine Homo sapiens
W397A no detectable activity with serotonin Homo sapiens
Y398F 3.5fold increase in Km-value for phenylethylamine, 7.9fold increase in IC50-value for clorgyline, 15.5fold increase in IC50-value for deprenyl Homo sapiens
Y398S no activity with phenylethylamine Homo sapiens
Y407F 1.4fold increase in Km-value for serotonin, 1.9fold increase in IC50-value for clorgyline, 10.9fold increase in IC50-value for deprenyl Homo sapiens
Y407S no detectable activity with serotonin Homo sapiens
Y435F 9.6fold increase in Km-value for phenylethylamine, 8.6fold increase in IC50-value for clorgyline, 130fold increase in IC50-value for deprenyl Homo sapiens
Y435S no activity with phenylethylamine Homo sapiens
Y444F activity with serotonin is too low to determine Km-value, 15.8fold increase in IC50-value for clorgyline, 10.4fold increase in IC50-value for deprenyl Homo sapiens
Y444S no detectable activity with serotonin Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Clorgyline IC50: 0.00042 mM; IC50: 1.2 nM Homo sapiens
Deprenyl IC50: 0.0023 mM; IC50: 3.3 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
 Phenylethylamine monoamine oxidase B wild-type enzyme Homo sapiens
0.0052
-
 Phenylethylamine monoamine oxidase B mutant D123A Homo sapiens
0.0078
-
 Phenylethylamine monoamine oxidase B mutant Y398F Homo sapiens
0.0211
-
 Phenylethylamine monoamine oxidase B mutant Y435F Homo sapiens
0.0863
-
 serotonin monoamine oxidase A wild-type enzyme Homo sapiens
0.12
-
 serotonin monoamine oxidase A mutant Y407F Homo sapiens
0.1866
-
 serotonin monoamine oxidase A mutant D132A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylethylamine + H2O + O2
-
 Homo sapiens 2-phenylethanal + NH3 + H2O2
-
 ?
serotonin + H2O + O2
-
 Homo sapiens (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
MAO A
-
 Homo sapiens
MAO B
-
 Homo sapiens
monoamine oxidase A
-
 Homo sapiens
monoamine oxidase B
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD Lys296, Trp388, and Tyr398 may be involved in the non-covalent binding to FAD Homo sapiens
FAD Lys305, Trp397, and Tyr407 may be involved in the non-covalent binding to FAD Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000012
-
 IC50: 1.2 nM Homo sapiens Clorgyline
0.00042
-
 IC50: 0.00042 mM Homo sapiens Clorgyline
0.0023
-
 IC50: 0.0023 mM Homo sapiens Deprenyl
3.3
-
 IC50: 3.3 mM Homo sapiens Deprenyl