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Literature summary for 1.4.3.4 extracted from

  • Newton-Vinson, P.; Hubalek, F.; Edmondson, D.E.
    High-level expression of human liver monoamine oxidase B in Pichia pastoris (2000), Protein Expr. Purif., 20, 334-345.
    View publication on PubMed
Search for more literature for 1.4.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(aminomethyl)trimethylsilane
-
 Homo sapiens
5-(aminomethyl)-3-aryl-2-oxazolidinones
-
 Homo sapiens
Pargyline MAO-B sensitive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens
additional information
-
 additional information
-
 Bos taurus
0.33
-
 O2
-
 Homo sapiens
0.5
-
 benzylamine
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial outer membrane
-
 Homo sapiens 5741
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59163
-
 x * 59163, electrospray mass spectroscopy, including one covalent FAD, MAO-B Homo sapiens
59474
-
 x * 60000, SDS-PAGE, x * 59474, electrospray mass spectroscopy, including one covalent FAD, MAO-B Homo sapiens
60000
-
 x * 60000, SDS-PAGE, x * 59474, electrospray mass spectroscopy, including one covalent FAD, MAO-B Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RCH2NH2 + H2O + O2 Bos taurus responsible for the catabolism of various biogenic amine neurotransmitters as well as for the metabolism of certain exogenous amines RCHO + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
additional information
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
considerable stability to handling and storage, MAO-B Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylamine + H2O + O2
-
 Homo sapiens benzaldehyde + NH3 + H2O2
-
 ?
RCH2NH2 + H2O + O2 responsible for the catabolism of various biogenic amine neurotransmitters as well as for the metabolism of certain exogenous amines Bos taurus RCHO + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 59163, electrospray mass spectroscopy, including one covalent FAD, MAO-B Homo sapiens
? x * 60000, SDS-PAGE, x * 59474, electrospray mass spectroscopy, including one covalent FAD, MAO-B Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
 benzylamine recombinant MAO-B Homo sapiens

Cofactor

Cofactor Comment Organism Structure
flavin 1 mol FAD per mol of MAO B is covalently bound by an 8-alpha-S-cysteinyl 397 linkage Homo sapiens