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Literature summary for 1.4.3.3 extracted from
- A novel thermostable D-amino acid oxidase of the thermophilic fungus Rasamsonia emersonii strain YA (2019), Sci. Rep., 9, 11948 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rasamsonia emersonii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-malate | 10 mM, 13.5% inhibition | Rasamsonia emersonii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
D-methionine | pH 8, 55°C | Rasamsonia emersonii | |
| 0.63 | - |
D-valine | pH 8, 55°C | Rasamsonia emersonii | |
| 2.7 | - |
D-alanine | pH 8, 55°C | Rasamsonia emersonii | |
| 12.1 | - |
D-glutamate | pH 8, 55°C | Rasamsonia emersonii | |
| 19.2 | - |
D-arginine | pH 8, 55°C | Rasamsonia emersonii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 185000 | - |
gel filtration | Rasamsonia emersonii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rasamsonia emersonii | A0A499UB99 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
substrate D-isoleucine, 37°C, pH 8.0 | Rasamsonia emersonii |
| 112 | - |
substrate D-isoleucine, 55°C, pH 8.0 | Rasamsonia emersonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cephalosporin C + H2O + O2 | - |
Rasamsonia emersonii | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
| D-alanine + H2O + O2 | - |
Rasamsonia emersonii | pyruvate + NH3 + H2O2 | - |
? | |
| D-allo-isoleucine + H2O + O2 | - |
Rasamsonia emersonii | (3R)-3-hydroxy-2-oxobutanoate + NH3 + H2O2 | - |
? | |
| D-allo-threonine + H2O + O2 | - |
Rasamsonia emersonii | ? + NH3 + H2O2 | - |
? | |
| D-arginine + H2O + O2 | - |
Rasamsonia emersonii | 5-guanidino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | - |
Rasamsonia emersonii | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
| D-glutamine + H2O + O2 | - |
Rasamsonia emersonii | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
| D-histidine + H2O + O2 | - |
Rasamsonia emersonii | 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2 | - |
? | |
| D-isoleucine + H2O + O2 | - |
Rasamsonia emersonii | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-leucine + H2O + O2 | - |
Rasamsonia emersonii | 4-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-methionine + H2O + O2 | - |
Rasamsonia emersonii | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
| D-phenylalanine + H2O + O2 | - |
Rasamsonia emersonii | phenylpyruvate + NH3 + H2O2 | - |
? | |
| D-serine + H2O + O2 | - |
Rasamsonia emersonii | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
| D-tyrosine + H2O + O2 | - |
Rasamsonia emersonii | 4-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
| D-valine + H2O + O2 | - |
Rasamsonia emersonii | 2-oxoisovalerate + NH3 + H2O2 | - |
? | |
| additional information | no substrates: L-isoleucine, L-valine, L-methionine | Rasamsonia emersonii | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 42000, SDS-PAGE | Rasamsonia emersonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Rasamsonia emersonii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable for 1 h | Rasamsonia emersonii |
| 60 | - |
T50 value | Rasamsonia emersonii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 45.6 | - |
D-arginine | pH 8, 55°C | Rasamsonia emersonii | |
| 90.7 | - |
D-glutamate | pH 8, 55°C | Rasamsonia emersonii | |
| 120 | - |
D-methionine | pH 8, 55°C | Rasamsonia emersonii | |
| 189 | - |
D-alanine | pH 8, 55°C | Rasamsonia emersonii | |
| 225 | - |
D-valine | pH 8, 55°C | Rasamsonia emersonii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rasamsonia emersonii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | stable for at least 1 h | Rasamsonia emersonii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Rasamsonia emersonii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.38 | - |
D-arginine | pH 8, 55°C | Rasamsonia emersonii | |
| 7.49 | - |
D-glutamate | pH 8, 55°C | Rasamsonia emersonii | |
| 69.9 | - |
D-alanine | pH 8, 55°C | Rasamsonia emersonii | |
| 358 | - |
D-valine | pH 8, 55°C | Rasamsonia emersonii | |
| 562 | - |
D-methionine | pH 8, 55°C | Rasamsonia emersonii | |
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