Literature summary for 1.4.3.3 extracted from

Rachadech, W.; Kato, Y.; Abou El-Magd, R.M.; Shishido, Y.; Kim, S.H.; Sogabe, H.; Maita, N.; Yorita, K.; Fukui, K.
P219L substitution in human D-amino acid oxidase impacts the ligand binding and catalytic efficiency (2020), J. Biochem., 168, 557-567 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of mutant P219L in complex with FAD and benzoate at 2.25 A resolution displays conformational changes of the active site and lid. The distances between the H-bond-forming atoms of arginine 283 and benzoate and the relative position between the aromatic rings of tyrosine 224 and benzoate are changed in the P219L complex	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
P219L	mutation to corresponding residue of porcine DAO. The turnover numbers (kcat) of P219L are unchanged, but its Km values are decreased compared with wild-type, leading to an increase in the catalytic efficiency (kcat/Km). Benzoate inhibits P219L with lower Ki value	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
benzoate	competitive	Homo sapiens
D-alanine	-	Homo sapiens
D-proline	-	Homo sapiens
D-serine	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.47	-	D-alanine	mutant P219L, pH 8.0, 25°C	Homo sapiens
0.73	-	D-proline	mutant P219L, pH 8.0, 25°C	Homo sapiens
0.75	-	D-alanine	wild-type, pH 8.0, 25°C	Homo sapiens
1.23	-	D-proline	wild-type, pH 8.0, 25°C	Homo sapiens
1.36	-	D-serine	mutant P219L, pH 8.0, 25°C	Homo sapiens
4.29	-	D-serine	wild-type, pH 8.0, 25°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P14920	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial purification by heat treatment at 53°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-alanine + H2O + O2	-	Homo sapiens	pyruvate + H2O2 + NH3	-	?
D-proline + H2O + O2	-	Homo sapiens	2-oxopentanoate + NH3 + H2O2	-	?
D-serine + H2O + O2	-	Homo sapiens	3-hydroxypyruvate + NH3 + H2O2	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.08	-	D-serine	mutant P219L, pH 8.0, 25°C	Homo sapiens
6.55	-	D-serine	wild-type, pH 8.0, 25°C	Homo sapiens
8.3	-	D-alanine	mutant P219L, pH 8.0, 25°C	Homo sapiens
9.48	-	D-alanine	wild-type, pH 8.0, 25°C	Homo sapiens
26.57	-	D-proline	wild-type, pH 8.0, 25°C	Homo sapiens
29.53	-	D-proline	mutant P219L, pH 8.0, 25°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0007	-	D-alanine	mutant P219L, pH 8.0, 25°C	Homo sapiens
0.0007	-	D-serine	mutant P219L, pH 8.0, 25°C	Homo sapiens
0.0009	-	D-proline	mutant P219L, pH 8.0, 25°C	Homo sapiens
0.0012	-	D-alanine	wild-type, pH 8.0, 25°C	Homo sapiens
0.0017	-	D-proline	wild-type, pH 8.0, 25°C	Homo sapiens
0.002	-	D-serine	wild-type, pH 8.0, 25°C	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.53	-	D-serine	wild-type, pH 8.0, 25°C	Homo sapiens
4.67	-	D-serine	mutant P219L, pH 8.0, 25°C	Homo sapiens
13.38	-	D-alanine	wild-type, pH 8.0, 25°C	Homo sapiens
18.52	-	D-alanine	mutant P219L, pH 8.0, 25°C	Homo sapiens
21.93	-	D-proline	wild-type, pH 8.0, 25°C	Homo sapiens
41.47	-	D-proline	mutant P219L, pH 8.0, 25°C	Homo sapiens

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Release 2023.1 (January 2023)