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Literature summary for 1.4.3.3 extracted from

  • Rosini, E.; Caldinelli, L.; Piubelli, L.
    Assays of D-amino acid oxidase activity (2017), Front. Mol. Biosci., 4, 102 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.3

Application

Application Comment Organism
analysis protocols for a variety of direct assays based on the determination of molecular oxygen consumption, reduction of alternative electron acceptors, or alpha-keto acid production, of coupled assays to detect the hydrogen peroxide or the ammonium production, and an indirect assay of the alpha-keto acid production based on a chemical derivatization Rhodotorula toruloides
analysis protocols for a variety of direct assays based on the determination of molecular oxygen consumption, reduction of alternative electron acceptors, or alpha-keto acid production, of coupled assays to detect the hydrogen peroxide or the ammonium production, and an indirect assay of the alpha-keto acid production based on a chemical derivatization Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 D-alanine pH 8, 25°C, oxygen consumption assay Rhodotorula toruloides
14.6
-
 D-Lysine pH 8, 25°C, oxygen consumption assay Rhodotorula toruloides
76.4
-
 D-glutamate pH 8, 25°C, oxygen consumption assay Rhodotorula toruloides

Organism

Organism UniProt Comment Textmining
Homo sapiens P14920
-
-
Rhodotorula toruloides P80324
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
 Rhodotorula toruloides pyruvate + H2O2 + NH3
-
 ?
D-glutamate + H2O + O2
-
 Rhodotorula toruloides 2-oxoglutarate + NH3 + H2O2
-
 ?
D-lysine + H2O + O2
-
 Rhodotorula toruloides 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
DAAO
-
 Rhodotorula toruloides
DAO1
-
 Rhodotorula toruloides