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Literature summary for 1.4.3.3 extracted from
- Catalytic and structural characteristics of carp hepatopancreas D-amino acid oxidase expressed in Escherichia coli (2005), Comp. Biochem. Physiol. B, 140, 417-425.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Cyprinus carpio |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzoate | 75 mM, 76% inhibition | Cyprinus carpio |  |
| creatinine | 75 mM, 86% inhibition | Cyprinus carpio | |
| HgCl2 | 0.1 mM, 71% inhibition | Cyprinus carpio | |
| PCMB | 75 mM, 93% inhibition | Cyprinus carpio | |
| Silver nitrate | 0.1 mM, 66% inhibition | Cyprinus carpio | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.226 | - |
D-Tyr | pH 8.0, 37°C | Cyprinus carpio | |
| 0.23 | - |
D-Ala | pH 8.0, 37°C | Cyprinus carpio | |
| 0.263 | - |
D-Val | pH 8.0, 37°C | Cyprinus carpio | |
| 0.357 | - |
D-Phe | pH 8.0, 37°C | Cyprinus carpio | |
| 0.425 | - |
D-Lys | pH 8.0, 37°C | Cyprinus carpio | |
| 0.909 | - |
D-Ser | pH 8.0, 37°C | Cyprinus carpio | |
| 1.06 | - |
D-Ile | pH 8.0, 37°C | Cyprinus carpio | |
| 1.13 | - |
D-Pro | pH 8.0, 37°C | Cyprinus carpio | |
| 6.5 | - |
D-Leu | pH 8.0, 37°C | Cyprinus carpio | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyprinus carpio | Q6TGN2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Cyprinus carpio |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Cyprinus carpio | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 293 | - |
- |
Cyprinus carpio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala + H2O + O2 | - |
Cyprinus carpio | pyruvate + NH3 + H2O2 | - |
? | |
| D-Ile + H2O + O2 | - |
Cyprinus carpio | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-Leu + H2O + O2 | - |
Cyprinus carpio | 4-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-Lys + H2O + O2 | - |
Cyprinus carpio | ? + NH3 + H2O2 | - |
? | |
| D-Phe + H2O + O2 | - |
Cyprinus carpio | phenylpyruvate + NH3 + H2O2 | - |
? | |
| D-Pro + H2O + O2 | - |
Cyprinus carpio | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-Ser + H2O + O2 | - |
Cyprinus carpio | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
| D-Tyr + H2O + O2 | - |
Cyprinus carpio | 4-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
| D-Val + H2O + O2 | - |
Cyprinus carpio | alpha-ketoisovalerate + NH3 + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chDAO | - |
Cyprinus carpio |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Cyprinus carpio |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 75% of maximal activity, 60°C: about 40% of maximal activity | Cyprinus carpio |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable below | Cyprinus carpio |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34.5 | - |
D-Ser | pH 8.0, 37°C | Cyprinus carpio | |
| 42.3 | - |
D-Lys | pH 8.0, 37°C | Cyprinus carpio | |
| 76.7 | - |
D-Ile | pH 8.0, 37°C | Cyprinus carpio | |
| 116 | - |
D-Leu | pH 8.0, 37°C | Cyprinus carpio | |
| 117 | - |
D-Tyr | pH 8.0, 37°C | Cyprinus carpio | |
| 119 | - |
D-Phe | pH 8.0, 37°C | Cyprinus carpio | |
| 142 | - |
D-Pro | pH 8.0, 37°C | Cyprinus carpio | |
| 170 | - |
D-Val | pH 8.0, 37°C | Cyprinus carpio | |
| 190 | - |
D-Ala | pH 8.0, 37°C | Cyprinus carpio | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Cyprinus carpio |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 11 | pH 6.5: about 75% of maximal activity, pH 11.0: about 75% of maximal activity | Cyprinus carpio |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 11 | 1 h, stable | Cyprinus carpio |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.1 | - |
creatinine | pH 8.0, 37°C | Cyprinus carpio | |
| 6 | - |
PCMB | pH 8.0, 37°C | Cyprinus carpio | |
| 12.5 | - |
benzoate | pH 8.0, 37°C | Cyprinus carpio | |
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