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Literature summary for 1.4.3.3 extracted from
- Isolation and characterization of a mitochondrial D-amino acid oxidase from Neurospora crassa (1977), Can. J. Biochem., 55, 66-74.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Neurospora crassa |  |
| p-chloromercuribenzoate | - |
Neurospora crassa | |
| Zn2+ | - |
Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
D-methionine | - |
Neurospora crassa | |
| 0.28 | - |
D-leucine | - |
Neurospora crassa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | matrix | Neurospora crassa | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 118000 | 125000 | PAGE, gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + O2 | Neurospora crassa | - |
pyruvic acid + NH3 + H2O2 | - |
? | |
| D-ethionine + H2O + O2 | Neurospora crassa | - |
4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2 | - |
? | |
| D-leucine + H2O + O2 | Neurospora crassa | - |
4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
| D-methionine + H2O + O2 | Neurospora crassa | - |
4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
| D-norleucine + H2O + O2 | Neurospora crassa | - |
2-oxohexanoate + NH3 + H2O2 | - |
? | |
| D-norvaline + H2O + O2 | Neurospora crassa | - |
2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-phenylalanine + H2O + O2 | Neurospora crassa | - |
phenylpyruvic acid + NH3 + H2O2 | - |
? | |
| D-valine + H2O + O2 | Neurospora crassa | - |
alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | - |
- |
Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + O2 | - |
Neurospora crassa | pyruvic acid + NH3 + H2O2 | - |
? | |
| D-ethionine + H2O + O2 | - |
Neurospora crassa | 4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2 | - |
? | |
| D-leucine + H2O + O2 | - |
Neurospora crassa | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
| D-methionine + H2O + O2 | - |
Neurospora crassa | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
| D-norleucine + H2O + O2 | - |
Neurospora crassa | 2-oxohexanoate + NH3 + H2O2 | - |
? | |
| D-norvaline + H2O + O2 | - |
Neurospora crassa | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-phenylalanine + H2O + O2 | - |
Neurospora crassa | phenylpyruvic acid + NH3 + H2O2 | - |
? | |
| D-valine + H2O + O2 | - |
Neurospora crassa | alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | - |
Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.2 | D-methionine | Neurospora crassa |
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