Cloned (Comment) | Organism |
---|---|
gene AOC1, expression of hDAO as a secreted enzyme in Drosophila S2 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type hDAO, hanging drop vapour diffusion, 200 nl protein solution, containing 10 mg/ml protein in 100 mM HEPES, pH 7.2, and 150 mM KCl, and 200 nl crystallant solution, containing 0.1 M MES, pH 6.5, and 12% w/v PEG 20000, are equilibrated over 0.075 ml reservoir solution at room temperature, eight weeks, method optimization using manually dispensed hanging-drop crystallization experiments containing 0.002 ml each of protein and crystallant equilibrated over 0.5 ml reservoir solution, best diffracting crystals grow using 0.1 M MES pH 6.1 and 12% w/v PEG at room temperature over a period of two months, X-ray diffraction structure determination and analysis at 2.1 A resolution | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | required, binding structure at the active site, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | hDAO is unique among CAOs in that it has a distinct substrate preference for diamines | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P19801 | gene AOC1 | - |
Purification (Comment) | Organism |
---|---|
recombinant secreted hDAO from Drosophila S2 cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hDAO is unique among CAOs in that it has a distinct substrate preference for diamines | Homo sapiens | ? | - |
? | |
additional information | active site of hDAO: contacts made by cofactor trihydroxyphenylalanine quinone to the active-site Cu2+, and Thr685 and to a glycerol molecule within the active-site channel | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | hDAO has the archetypal CAO fold with each subunit of hDAO being comprised of domains D2, residues 27-135, D3, residues 144-258, and D4, residues 310-751. The domains are linked by loop regions consisting of residues 136-143 between D2 and D3 and residues 259-309 linking D3 to D4, structure and modelling, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DAO | - |
Homo sapiens |
More | the enzyme is a member of the copper-containing amine oxidase, CAO, family of enzymes | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
trihydroxyphenylalanine quinone | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | hDAO is the frontline enzyme for histamine metabolism | Homo sapiens |