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Literature summary for 1.4.3.22 extracted from
- Purification and properties of the diamine oxidase from Vicia faba leaves (1981), Plant Cell Physiol., 22, 737-746.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,4-phenanthroline | 0.1 mM | Vicia faba |  |
| Cuprizone | 0.1 mM | Vicia faba | |
| diethyldithiocarbamate | - |
Vicia faba | |
| phenylhydrazine | - |
Vicia faba | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | 2 copper atoms per mol of enzyme | Vicia faba | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
2 * 74000, SDS-PAGE | Vicia faba |
| 126000 | - |
gel filtration | Vicia faba |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vicia faba | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vicia faba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Vicia faba | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.6 | - |
- |
Vicia faba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-diaminobutane + H2O + O2 | - |
Vicia faba | 4-aminobutanal + NH3 + H2O2 | - |
? | |
| 1,5-diaminopentane + H2O + O2 | - |
Vicia faba | 5-aminopentanal + NH3 + H2O2 | - |
? | |
| 2-phenylethylamine + H2O + O2 | - |
Vicia faba | 2-phenylethanal + NH3 + H2O2 | - |
? | |
| agmatine | - |
Vicia faba | ? | - |
? | |
| benzylamine + H2O + O2 | - |
Vicia faba | benzaldehyde + NH3 + H2O2 | - |
? | |
| histamine + H2O + O2 | - |
Vicia faba | (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
| lysine + H2O + O2 | - |
Vicia faba | 2-aminohexanoic acid + NH3 + H2O2 | - |
? | |
| RCH2NH2 + H2O + O2 | - |
Vicia faba | RCHO + NH3 + H2O2 | - |
? | |
| spermidine + H2O + O2 | - |
Vicia faba | ? | - |
? | |
| spermine + H2O + O2 | - |
Vicia faba | ? | - |
? | |
| tryptamine | - |
Vicia faba | ? | - |
? | |
| tyramine + H2O + O2 | - |
Vicia faba | 4-hydroxyphenylethanal + NH3 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 74000, SDS-PAGE | Vicia faba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Vicia faba |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 63 | about 50% activity at 20°C and 63°C | Vicia faba |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
60 | 10 min, pH 7.0, stable | Vicia faba |
| 70 | - |
10 min, pH 7, 100% loss of activity | Vicia faba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
cadaverine, histamine, agmatine | Vicia faba |
| 7.2 | - |
putrescine, spermidine, tryptamine | Vicia faba |
| 7.7 | - |
tyramine | Vicia faba |
| 8.5 | - |
dopamine, phenylethylamine, benzylamine | Vicia faba |
| 8.6 | - |
lysine | Vicia faba |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
4 h, complete loss of activity | Vicia faba |
| 3.5 | - |
4 h, 50% loss of activity | Vicia faba |
| 5 | 9 | 30°C, stable | Vicia faba |
| 11 | - |
4 h, 50% loss of activity | Vicia faba |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 1 mol carbonyl-group per mol enzyme | Vicia faba |
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