Literature summary for 1.4.3.22 extracted from

  • Rinaldi, A.; Floris, G.; Finazzi-Agro, A.
    Purification and properties of diamine oxidase from Euphorbia latex (1982), Eur. J. Biochem., 127, 417-422.
    View publication on PubMed

General Stability

General Stability Organism
purified enzyme does not withstand lyophilization Euphorbia characias

Inhibitors

Inhibitors Comment Organism Structure
Arcaine sulfate
-
Euphorbia characias
cyanide
-
Euphorbia characias
diethyldithiocarbamate
-
Euphorbia characias
Diethylthiocarbamate
-
Euphorbia characias
histamine
-
Euphorbia characias
isoniazid
-
Euphorbia characias
NaCN
-
Euphorbia characias
NaN3
-
Euphorbia characias
phenylhydrazine
-
Euphorbia characias
Semicarbazide
-
Euphorbia characias

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
putrescine
-
Euphorbia characias
0.2
-
1,4-diaminobutane
-
Euphorbia characias
0.47
-
cadaverine
-
Euphorbia characias
0.47
-
1,5-Diaminopentane
-
Euphorbia characias

Metals/Ions

Metals/Ions Comment Organism Structure
copper enzyme contains 2 Cu2+ per dimer Euphorbia characias
Manganese no manganese found Euphorbia characias

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
gel filtration Euphorbia characias
72000
-
2 * 72000, SDS-PAGE Euphorbia characias

Organism

Organism UniProt Comment Textmining
Euphorbia characias
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 12% neutral sugar Euphorbia characias

Purification (Commentary)

Purification (Comment) Organism
-
Euphorbia characias

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
-
Euphorbia characias

Storage Stability

Storage Stability Organism
-20°C, in water or potassium phosphate buffer, indefinitely stable Euphorbia characias
4°C, 50% loss of activity after 15 days Euphorbia characias

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RCH2NH2 + H2O + O2 no activity with spermidine Euphorbia characias RCHO + NH3 + H2O2
-
?
RCH2NH2 + H2O + O2 no activity with benzylamine Euphorbia characias RCHO + NH3 + H2O2
-
?
RCH2NH2 + H2O + O2 no activity with p-dimethylaminomethylbenzylamine Euphorbia characias RCHO + NH3 + H2O2
-
?
RCH2NH2 + H2O + O2 no activity with histamine Euphorbia characias RCHO + NH3 + H2O2
-
?
RCH2NH2 + H2O + O2 only aliphatic diamines from C4 to C10 and cystamine are oxidized, enzymatic activity decreases sharply with increasing chain length of diamines Euphorbia characias RCHO + NH3 + H2O2
-
?
1,4-diaminobutane + H2O + O2
-
Euphorbia characias 4-aminobutanal + NH3 + H2O2
-
?
1,5-diaminopentane + H2O + O2
-
Euphorbia characias 5-aminopentanal + NH3 + H2O2
-
?
1,6-diaminohexane + H2O + O2
-
Euphorbia characias 6-aminohexanal + NH3 + H2O2
-
?
1,7-diaminoheptane + H2O + O2
-
Euphorbia characias 7-aminoheptanal + NH3 + H2O2
-
?
1,10-diaminodecane + H2O + O2
-
Euphorbia characias 10-aminodecanal + NH3 + H2O2
-
?
spermine + H2O + O2 no reaction Euphorbia characias ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 72000, SDS-PAGE Euphorbia characias

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
in Tris buffer Euphorbia characias
7.1
-
phosphate buffer Euphorbia characias

Cofactor

Cofactor Comment Organism Structure
additional information contains 2 carbonyl-like groups per dimer Euphorbia characias

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.12
-
NaCN
-
Euphorbia characias
0.05
-
Semicarbazide
-
Euphorbia characias
0.00025
-
phenylhydrazine
-
Euphorbia characias
6
-
histamine
-
Euphorbia characias
6
-
isoniazid
-
Euphorbia characias
0.004
-
Arcaine sulfate
-
Euphorbia characias
0.43
-
Diethylthiocarbamate
-
Euphorbia characias
54
-
NaN3
-
Euphorbia characias