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Literature summary for 1.4.3.21 extracted from
- Structural analysis of aliphatic versus aromatic substrate specificity in a copper amine oxidase from Hansenula polymorpha (2013), Biochemistry, 52, 2291-2301.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae | Ogataea angusta |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with ethylamine and benzylamine, hanging drop vapor diffusion method, using 8-9% (w/v) polyethylene glycol 8000 in 0.22-0.25 M potassium phosphate (pH 6.0) | Ogataea angusta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Ogataea angusta | the catalytic mechanism can be divided into two half-reactions: a reductive half-reaction in which a primary amine substrate is oxidized to its corresponding aldehyde with the concomitant reduction of the organic cofactor 2,4,5-trihydroxyphenylalanine quinone and an oxidative half-reaction in which reduced 2,4,5-trihydroxyphenylalanine quinone is re-oxidized with the reduction of molecular oxygen to hydrogen peroxide | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | P12807 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzylamine + H2O + O2 | - |
Ogataea angusta | benzaldehyde + NH3 + H2O2 | - |
? | |
| ethylamine + H2O + O2 | - |
Ogataea angusta | acetaldehyde + NH3 + H2O2 | - |
? | |
| additional information | the catalytic mechanism can be divided into two half-reactions: a reductive half-reaction in which a primary amine substrate is oxidized to its corresponding aldehyde with the concomitant reduction of the organic cofactor 2,4,5-trihydroxyphenylalanine quinone and an oxidative half-reaction in which reduced 2,4,5-trihydroxyphenylalanine quinone is re-oxidized with the reduction of molecular oxygen to hydrogen peroxide | Ogataea angusta | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAO | - |
Ogataea angusta |
| Copper amine oxidase | - |
Ogataea angusta |
| hPAO-1 | - |
Ogataea angusta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
benzylamine | in 100 mM potassium phosphate, pH 7.2, temperature not specified in the publication | Ogataea angusta | |
| 20 | - |
ethylamine | in 100 mM potassium phosphate, pH 7.2, temperature not specified in the publication | Ogataea angusta | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,4,5-trihydroxyphenylalanine quinone | - |
Ogataea angusta | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
benzylamine | in 100 mM potassium phosphate, pH 7.2, temperature not specified in the publication | Ogataea angusta | |
| 52 | - |
ethylamine | in 100 mM potassium phosphate, pH 7.2, temperature not specified in the publication | Ogataea angusta | |
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Release 2023.1 (January 2023)
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