Crystallization (Comment) | Organism |
---|---|
purified recombinant C-terminal StrepII-tagged enzyme in complex with inhibitors benzylhydrazine or tranylcypromine, vapour diffusion in hanging drop method, mixing of protein solution containing about 10 mg/ml protein in 50 mM HEPES, pH 7.0, with well solution containing 1.6 M ammonium sulfate and 150 mM sodium citrate pH 7.0. CuSO4, in a twofold molar excess, 2 weeks. The crystals are then transferred to a sitting drop well solution containing 30% v/v glycerol and 2 mM benzylhydrazine dihydrochloride or 0.4 mM tranylcypromine for 30 min, X-ray diffraction structure determination and analysis at 1.65-1.86 A resolution | Arthrobacter globiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzylhydrazine | forms adducts with the TPQ cofactor, binding structure, overview | Arthrobacter globiformis | |
tranylcypromine | forms adducts with the TPQ cofactor, also termed (1R,2S)-rel-2-phenylcyclopropanamine, is a mixture of (1R,2S)-2-phenylcyclopropanamine and (1S,2R)-2-phenylcyclopropanamine, binding structure, overview | Arthrobacter globiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | bound by three His ligands of the active-site | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | P46881 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
copper-containing amine oxidase | - |
Arthrobacter globiformis |
quinone-containing copper amine oxidase | - |
Arthrobacter globiformis |
TPQ-containing CuAO | - |
Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,4,5-trihydroxyphenylalaninequinone | i.e. TPQ cofactor, the cofactor is spontaneously formed by post-translational modifications of active site amino-acid residues | Arthrobacter globiformis |