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Literature summary for 1.4.3.21 extracted from

  • Carper, W.R.; Stoddard, D.D.; Martin, D.F.
    Pig liver monoamine oxidase I: isolation and characterization (1974), Biochim. Biophys. Acta, 334, 287-296.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
1,4-phenanthroline 0.33 mM, 65% inhibition Sus scrofa
diethyldithiocarbamate 3.3 mM, 74% inhibition Sus scrofa
hydroxylamine 3.3 mM, 30% inhibition Sus scrofa
NaN3 3.3 mM, 48% inhibition Sus scrofa
Neocuproine 0.033 mM, 61% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00128
-
benzylamine at pH 9.0 Sus scrofa
0.0174
-
benzylamine at pH 7.2 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Cu2+ in the enzyme Sus scrofa
copper contains 8 Cu2+ per 1200000 Da, Co2+, Zn2+ and Ni2+ can replace Cu2+, no effect of Mn2+ Sus scrofa
copper copper protein Sus scrofa
Ni2+ can replace Cu2+ in the enzyme Sus scrofa
Zn2+ can replace Cu2+ in the enzyme Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
146000
-
8 * 146000 Sus scrofa
1200000
-
gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
1100.0-1200.0 units/mg, 1 unit is defined as the amount of enzyme catalyzing a change of 0.001 absorbance per minute at 25°C Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylamine + H2O + O2
-
Sus scrofa benzaldehyde + NH3 + H2O2
-
?
RCH2NH2 + H2O + O2
-
Sus scrofa RCHO + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
octamer 8 * 146000 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 relative activity at 20°C: 22.8, at 40°C: 31.2 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
50 mM glycine, 1 mM EDTA Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Sus scrofa