Literature summary for 1.4.3.2 extracted from

Salama, W.H.; Ibrahim, N.M.; El Hakim, A.E.; Bassuiny, R.I.; Mohamed, M.M.; Mousa, F.M.; Ali, M.M.
L-Amino acid oxidase from Cerastes vipera snake venom Isolation, characterization and biological effects on bacteria and tumor cell lines (2018), Toxicon, 150, 270-279 .

Search for more literature for 1.4.3.2

Application

Application	Comment	Organism
medicine	LAAO exhibits a considerable cytotoxic activity against breast cancer cell line MCF-7 with IC50 value of 2.75 microg/ml. LAAO triggers antiproliferative activity via extensive H2O2 generation. LAAO exhibits a significant bactericidal activity against gram-positive and gram-negative bacteria, particularly Staphylococcus aureus and Escherichia coli with MIC values of 20 microg/ml	Cerastes vipera

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	-	Cerastes vipera
Cu2+	-	Cerastes vipera
Hg2+	-	Cerastes vipera
additional information	not inhibitory: EDTA	Cerastes vipera
Ni2+	-	Cerastes vipera

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
12	-	L-leucine	pH 8, 37°C	Cerastes vipera

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	5 mM, enhances the enzyme activity by 10%	Cerastes vipera

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
120000	-	gel filtration	Cerastes vipera

Organism

Organism	UniProt	Comment	Textmining
Cerastes vipera	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration followed by anion exchange chromatography	Cerastes vipera

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Cerastes vipera	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-leucine + H2O + O2	-	Cerastes vipera	2-oxo-4-methylpentanoate + NH3 + H2O2	-	?
L-methionine + H2O + O2	best substrate	Cerastes vipera	4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2	-	?
L-phenylalanine + O2	-	Cerastes vipera	phenylpyruvate + NH3 + H2O2	-	?
additional information	activity on amino acids in descending order is L-Met > L-Phe > L-Tyr > L-Arg > L-Ile > L-Trp	Cerastes vipera	?	-	-

Subunits

Subunits	Comment	Organism
dimer	2 * 60000, SDS-PAGE	Cerastes vipera

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Cerastes vipera

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
60	-	25% residual activity	Cerastes vipera

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
12	-	L-leucine	pH 8, 37°C	Cerastes vipera

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Cerastes vipera

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Release 2023.1 (January 2023)