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Literature summary for 1.4.3.2 extracted from

  • Lu, W.; Hu, L.; Yang, J.; Sun, X.; Yan, H.; Liu, J.; Chen, J.; Cheng, X.; Zhou, Q.; Yu, Y.; Wei, J.F.; Cao, P.
    Isolation and pharmacological characterization of a new cytotoxic L-amino acid oxidase from Bungarus multicinctus snake venom (2018), J. Ethnopharmacol., 213, 311-320 .
    View publication on PubMed
Search for more literature for 1.4.3.2

Application

Application Comment Organism
medicine apotxin can selectively kill tumor cells, with less cytotoxicity to the normal cells. Its anti-tumor activity is mainly due to the hydrogen peroxide produced from LAAO oxidation, but catalase does not reverse its anti-tumor effect completely. Deglycosylation can significantly reduce the LAAO activity and anti-tumor activity of apotxin Bungarus multicinctus

Organism

Organism UniProt Comment Textmining
Bungarus multicinctus A8QL51
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-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sugars account for approximately 12% of total mass Bungarus multicinctus

Purification (Commentary)

Purification (Comment) Organism
by Sephadex G-75 gel filtration, Sephadex G-25 desalting, Q ion-exchange chromatography Bungarus multicinctus

Storage Stability

Storage Stability Organism
-20°C, 1 month, 93% residual activity Bungarus multicinctus
4°C, 1 month, 76% residual activity Bungarus multicinctus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + H2O + O2 about 70% of the activity with L-leucine Bungarus multicinctus 3-methyl-2-oxo-pentanoate + NH3 + H2O2
-
 ?
L-leucine + H2O + O2
-
 Bungarus multicinctus 4-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
L-methionine + H2O + O2 about 110% of the activity with L-leucine Bungarus multicinctus 4-(methylsulfanyl)-2-oxobutanoate + NH3 + H2O2
-
 ?
L-phenylalanine + O2 about 80% of the activity with L-leucine Bungarus multicinctus phenylpyruvate + NH3 + H2O2
-
 ?
L-tyrosine + H2O + O2 about 60% of the activity with L-leucine Bungarus multicinctus 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
additional information enzyme displays a catalytic preference for hydrophobic L-amino acids. It is very active against L-Met, L-Leu, L-Phe, L-Ile, L-Tyr and moderately active against L-Pro, L-Val, L-Gln and L-Ala while it has low catalytic activity for the other L-amino acids. No substrate: D-amino acids Bungarus multicinctus ?
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-

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE, x * 58800, calculated from sequence Bungarus multicinctus

Synonyms

Synonyms Comment Organism
apotxin
-
 Bungarus multicinctus
LAAO
-
 Bungarus multicinctus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bungarus multicinctus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 48
-
 Bungarus multicinctus