Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | about 5timer higher activity in the presence of 3 mM 4-chloromercuribenzoate | Pseudomonas sp. AIU 813 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. AIU 813 |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 8% (w/v) polyethylene glycol 4000 and 0.1 M Na-acetate (pH 4.6) | Pseudomonas sp. AIU 813 |
Protein Variants | Comment | Organism |
---|---|---|
C254I | the mutant enzyme shows 5times higher specific activity of oxidase activity than that of the wild type enzyme | Pseudomonas sp. AIU 813 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + H2O + O2 | Pseudomonas sp. AIU 813 | - |
5-guanidino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-Lys + H2O + O2 | Pseudomonas sp. AIU 813 | - |
6-amino-2-oxo-hexanoic acid + NH3 + H2O2 | - |
? | |
L-Orn + H2O + O2 | Pseudomonas sp. AIU 813 | - |
5-amino-2-oxopentanoic acid + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. AIU 813 | W6JQJ6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Pseudomonas sp. AIU 813 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + H2O + O2 | - |
Pseudomonas sp. AIU 813 | 5-guanidino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-Lys + H2O + O2 | - |
Pseudomonas sp. AIU 813 | 6-amino-2-oxo-hexanoic acid + NH3 + H2O2 | - |
? | |
L-Orn + H2O + O2 | - |
Pseudomonas sp. AIU 813 | 5-amino-2-oxopentanoic acid + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-AAO/MOG | - |
Pseudomonas sp. AIU 813 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas sp. AIU 813 |