Literature summary for 1.4.3.2 extracted from

Hou, Y.; Hossain, G.; Li, J.; Shin, H.D.; Du, G.; Liu, L.
Combination of phenylpyruvic acid (PPA) pathway engineering and molecular engineering of L-amino acid deaminase improves PPA production with an Escherichia coli whole-cell biocatalyst (2016), Appl. Microbiol. Biotechnol., 100, 2183-2191.

Search for more literature for 1.4.3.2

Application

Application	Comment	Organism
synthesis	engineering Escherichia coli to produce phenylpyruvate. Knock-out of three aminotransferases increases the phenylpyuvate titer from 3.3 to 3.9 g/l and the substrate conversion ratio to 97.5%. The L-amino acid deaminase triple mutant D165K/F263M/L336M produces 10.0 g phenylpyruvate per l, with a substrate conversion ratio of 100%. An optimal fed-batch biotransformation process gives 21 g phenylpyruvate per l within 8 h	Proteus mirabilis

Cloned(Commentary)

Cloned (Comment)	Organism
xcpression in Escherichia coli	Proteus mirabilis

Protein Variants

Protein Variants	Comment	Organism
D165K	slight increase in kcat/KM value for phenylpyruvate	Proteus mirabilis
D165K/F263M	slight increase in kcat/KM value for phenylpyruvate	Proteus mirabilis
D165K/F263M/L336	2fold increase in kcat/KM value for phenylpyruvate	Proteus mirabilis
D165K/L336M	slight increase in kcat/KM value for phenylpyruvate	Proteus mirabilis
D165K/S179L/F263V/L336V	slight increase in kcat/KM value for phenylpyruvate	Proteus mirabilis
F263M	slight increase in kcat/KM value for phenylpyruvate	Proteus mirabilis
F263M/L336M	2fold increase in kcat/KM value for phenylpyruvate	Proteus mirabilis
L336M	slight increase in kcat/KM value for phenylpyruvate	Proteus mirabilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
22	-	L-phenylalanine	mutant D165K/F263M/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
22.4	-	L-phenylalanine	mutant F263M/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
22.6	-	L-phenylalanine	mutant D165K/F263M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
23.8	-	L-phenylalanine	mutant F263M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
24.1	-	L-phenylalanine	mutant D165K/S179L/F263V/L336V, pH 7.0, temperature not specified in the publication	Proteus mirabilis
24.3	-	L-phenylalanine	mutant D165K/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
24.5	-	L-phenylalanine	mutant L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
24.8	-	L-phenylalanine	mutant D165K, pH 7.0, temperature not specified in the publication	Proteus mirabilis
26.2	-	L-phenylalanine	wild-type, pH 7.0, temperature not specified in the publication	Proteus mirabilis

Organism

Organism	UniProt	Comment	Textmining
Proteus mirabilis	-	-	-
Proteus mirabilis KCTC 2566	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-phenylalanine + H2O + O2	-	Proteus mirabilis	phenylpyruvate + NH3 + H2O2	-	?
L-phenylalanine + H2O + O2	-	Proteus mirabilis KCTC 2566	phenylpyruvate + NH3 + H2O2	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.4	-	L-phenylalanine	wild-type, pH 7.0, temperature not specified in the publication	Proteus mirabilis
1.67	-	L-phenylalanine	mutant D165K, pH 7.0, temperature not specified in the publication	Proteus mirabilis
1.69	-	L-phenylalanine	mutant L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
1.72	-	L-phenylalanine	mutant D165K/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
1.82	-	L-phenylalanine	mutant D165K/S179L/F263V/L336V, pH 7.0, temperature not specified in the publication	Proteus mirabilis
1.87	-	L-phenylalanine	mutant F263M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
2.16	-	L-phenylalanine	mutant D165K/F263M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
2.21	-	L-phenylalanine	mutant F263M/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
2.25	-	L-phenylalanine	mutant D165K/F263M/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.053	-	L-phenylalanine	wild-type, pH 7.0, temperature not specified in the publication	Proteus mirabilis
0.067	-	L-phenylalanine	mutant D165K, pH 7.0, temperature not specified in the publication	Proteus mirabilis
0.069	-	L-phenylalanine	mutant L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
0.071	-	L-phenylalanine	mutant D165K/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
0.076	-	L-phenylalanine	mutant D165K/S179L/F263V/L336V, pH 7.0, temperature not specified in the publication	Proteus mirabilis
0.079	-	L-phenylalanine	mutant F263M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
0.096	-	L-phenylalanine	mutant D165K/F263M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
0.099	-	L-phenylalanine	mutant F263M/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis
0.102	-	L-phenylalanine	mutant D165K/F263M/L336M, pH 7.0, temperature not specified in the publication	Proteus mirabilis

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Release 2023.1 (January 2023)