Crystallization (Comment) | Organism |
---|---|
purified native enzyme by sitting drop vapour diffusion method, using a protein concentration of 10 mg/ml, with 0.04 M zinc acetate and 16% w/v PEG 3350 as the precipitant, X-ray diffraction structure determination and analysis at 2.57 A resolution | Vipera ammodytes ammodytes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Vipera ammodytes ammodytes | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | one Zn2+ ion is anchored to His75 at the beta-hairpin formed by the segment 73-86, which stabilizes the oligomeric state by bridging His75 and Glu279 of the adjacent protomer | Vipera ammodytes ammodytes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vipera ammodytes ammodytes | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | an N-acetylglucosamine group bound to the Asn172 side chain | Vipera ammodytes ammodytes |
Purification (Comment) | Organism |
---|---|
native enzyme from venom by two repeated steps of anion exchange chromatography | Vipera ammodytes ammodytes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Vipera ammodytes ammodytes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | active site and substrate binding structure, overview | Vipera ammodytes ammodytes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | LAAO comprises the FAD-binding domain, the substrate binding domain, and the alpha-helical domain, the substrate binding domain is made up by residues 5-25, 73-129, 233-236 and 323-420, and contains 4 alpha-helices, formed by residues 16-25, 96-104, 375-383 and 386-401, and 11 beta-strands, residues 74-77, 82-85, 109-112, 120-123, 126-129, 233-236, 324-331, 345-347, 354-356, 367-374 and 412-419 | Vipera ammodytes ammodytes |
Synonyms | Comment | Organism |
---|---|---|
LAAO | - |
Vipera ammodytes ammodytes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the FAD-binding domain includes residues 35-72, 239-318 and 446-486. The secondary structure elements of this domain are 4 alpha-helices | Vipera ammodytes ammodytes |