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Literature summary for 1.4.3.2 extracted from

  • Jin, Y.; Lee, W.H.; Zeng, L.; Zhang, Y.
    Molecular characterization of L-amino acid oxidase from king cobra venom (2007), Toxicon, 50, 479-489.
    View publication on PubMed

Application

Application Comment Organism
medicine LAAO does not induce platelet aggregation, but it has potent inhibitory activity on platelet aggregation induced by ADP and U46619. It shows no effect on platelet aggregation induced by thrombin, mucetin, ristocetin and stejnulxin Ophiophagus hannah

Cloned(Commentary)

Cloned (Comment) Organism
-
Bungarus fasciatus
-
Bungarus multicinctus
-
Naja atra
into pGEM-T vector Ophiophagus hannah

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
L-methionine
-
Ophiophagus hannah
2.2
-
L-leucine
-
Ophiophagus hannah
2.3
-
L-histidine
-
Ophiophagus hannah
2.6
-
L-lysine
-
Ophiophagus hannah
2.9
-
L-isoleucine
-
Ophiophagus hannah

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53686
-
1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis Ophiophagus hannah
64000
-
1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis Ophiophagus hannah
67000
-
1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis Ophiophagus hannah

Organism

Organism UniProt Comment Textmining
Bungarus fasciatus A8QL52
-
-
Bungarus multicinctus A8QL51
-
-
Naja atra A8QL58
-
-
Ophiophagus hannah P81383
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, ion-exchange and heparin chromatography, to homogeneity Ophiophagus hannah

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Ophiophagus hannah
-
venom
-
Bungarus fasciatus
-
venom
-
Bungarus multicinctus
-
venom
-
Naja atra
-
venom gland
-
Ophiophagus hannah
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidine + H2O + O2
-
Ophiophagus hannah 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
?
L-isoleucine + H2O + O2
-
Ophiophagus hannah 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-leucine + H2O + O2
-
Ophiophagus hannah 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-lysine + H2O + O2
-
Ophiophagus hannah 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
L-methionine + H2O + O2
-
Ophiophagus hannah 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis Ophiophagus hannah

Synonyms

Synonyms Comment Organism
LAAO
-
Ophiophagus hannah
LAAO
-
Bungarus fasciatus
LAAO
-
Bungarus multicinctus
LAAO
-
Naja atra