Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, SDS-PAGE | Bothrops pirajai |
130000 | - |
gel filtration | Bothrops pirajai |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bothrops pirajai | enzyme induces time-dependent platelet aggregation, mouse paw edema, shows cytotoxic activity against Escherichia coli, Pseudomonas aeruginosa, Leishmania sp., and tumor cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops pirajai | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation does not alter enzymatic activity | Bothrops pirajai |
Purification (Comment) | Organism |
---|---|
- |
Bothrops pirajai |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops pirajai | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
300 | - |
substrate L-histidine, 25°C, pH 7.2 | Bothrops pirajai |
410 | - |
substrate L-valine, 25°C, pH 7.2 | Bothrops pirajai |
435 | - |
substrate L-isoleucine, 25°C, pH 7.2 | Bothrops pirajai |
440 | - |
substrate L-methionine, 25°C, pH 7.2 | Bothrops pirajai |
580 | - |
substrate L-leucine, 25°C, pH 7.2 | Bothrops pirajai |
585 | - |
substrate L-tryptophan, 25°C, pH 7.2 | Bothrops pirajai |
685 | - |
substrate L-tyrosine, 25°C, pH 7.2 | Bothrops pirajai |
765 | - |
substrate L-phenylalanine, 25°C, pH 7.2 | Bothrops pirajai |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidine + H2O + O2 | substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine | Bothrops pirajai | 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2 | - |
? | |
L-isoleucine + H2O + O2 | substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine | Bothrops pirajai | 3-methyl-2-oxo-pentanoic acid + NH3 + H2O2 | - |
? | |
L-leucine + H2O + O2 | substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine | Bothrops pirajai | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine | Bothrops pirajai | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine | Bothrops pirajai | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine | Bothrops pirajai | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
L-tyrosine + H2O + O2 | substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine | Bothrops pirajai | p-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
L-valine + H2O + O2 | substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine | Bothrops pirajai | 2-oxoisovalerate + NH3 + H2O2 | - |
? | |
additional information | enzyme induces time-dependent platelet aggregation, mouse paw edema, shows cytotoxic activity against Escherichia coli, Pseudomonas aeruginosa, Leishmania sp., and tumor cells | Bothrops pirajai | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 66000, SDS-PAGE | Bothrops pirajai |
Synonyms | Comment | Organism |
---|---|---|
Bpir-LAOO-1 | - |
Bothrops pirajai |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bothrops pirajai |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.4 | - |
Bothrops pirajai |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bothrops pirajai | isoelectric focusing | - |
4.9 |