Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.19 extracted from

  • Zhan, T.; Zhang, K.; Chen, Y.; Lin, Y.; Wu, G.; Zhang, L.; Yao, P.; Shao, Z.; Liu, Z.
    Improving glyphosate oxidation activity of glycine oxidase from Bacillus cereus by directed evolution (2013), PLoS ONE, 8, e79175 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus cereus

Protein Variants

Protein Variants Comment Organism
BG51R/G60S/I198V/E357G the mutant shows no activity with glycine and high activity with glyphosate Bacillus cereus
BG51R/G60S/S210P/M267T the mutant shows no activity with glycine and activity with glyphosate Bacillus cereus
D60G the mutant shows activity with glycine and very low activity with glyphosate Bacillus cereus
G51R the mutant shows no activity with glycine and very low activity with glyphosate Bacillus cereus
G51R/D60G the mutant shows no activity with glycine and activity with glyphosate Bacillus cereus
G51R/D60G/K133R/V262I the mutant shows no activity with glycine and activity with glyphosate Bacillus cereus
G51R/D60G/T118A/K133R the mutant shows no activity with glycine and activity with glyphosate Bacillus cereus
G51R/D60G/T118A/K133R/I284L the mutant shows no activity with glycine and very high activity with glyphosate Bacillus cereus
G51R/G60S/D121G/S122P/H164Y/M267T the mutant shows no activity with glycine and high activity with glyphosate Bacillus cereus
G51R/G60S/K133R/S210P/R250G/V262I the mutant shows no activity with glycine and high activity with glyphosate Bacillus cereus
G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G the mutant shows no activity with glycine and high activity with glyphosate Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
 glyphosate mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C Bacillus cereus
0.57
-
 glyphosate mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C Bacillus cereus
0.99
-
 glycine mutant enzyme D60G, at pH 8.5 and 60°C Bacillus cereus
1.04
-
 glycine wild type enzyme, at pH 8.5 and 60°C Bacillus cereus
1.37
-
 glyphosate mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C Bacillus cereus
1.69
-
 glyphosate mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C Bacillus cereus
2.17
-
 glyphosate mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C Bacillus cereus
2.45
-
 glyphosate mutant enzyme G51R/D60G, at pH 8.5 and 60°C Bacillus cereus
2.77
-
 glyphosate mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C Bacillus cereus
3.8
-
 glyphosate mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C Bacillus cereus
4.37
-
 glyphosate mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C Bacillus cereus
8.29
-
 glyphosate mutant enzyme G51R, at pH 8.5 and 60°C Bacillus cereus
18.88
-
 glyphosate mutant enzyme D60G, at pH 8.5 and 60°C Bacillus cereus
41.55
-
 glycine mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C Bacillus cereus
41.64
-
 glycine mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C Bacillus cereus
54.6
-
 glycine mutant enzyme G51R, at pH 8.5 and 60°C Bacillus cereus
58.5
-
 glycine mutant enzyme G51R/D60G, at pH 8.5 and 60°C Bacillus cereus
80.43
-
 glycine mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C Bacillus cereus
84.79
-
 glyphosate wild type enzyme, at pH 8.5 and 60°C Bacillus cereus
92.5
-
 glycine mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C Bacillus cereus
101.8
-
 glycine mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C Bacillus cereus
105.6
-
 glycine mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C Bacillus cereus
134.4
-
 glycine mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C Bacillus cereus
138.1
-
 glycine mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-iminoacetate + H2O Bacillus cereus
-
 glyoxylate + NH3
-
 ?
glycine + H2O + O2 Bacillus cereus
-
 glyoxylate + NH3 + H2O2
-
 ?
glycine + O2 Bacillus cereus
-
 2-iminoacetate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus L7T8Y9
-
-

Purification (Commentary)

Purification (Comment) Organism
GST-bind resin column chromatography Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-iminoacetate + H2O
-
 Bacillus cereus glyoxylate + NH3
-
 ?
glycine + H2O + O2
-
 Bacillus cereus glyoxylate + NH3 + H2O2
-
 ?
glycine + O2
-
 Bacillus cereus 2-iminoacetate + H2O2
-
 ?
glyphosate + H2O + O2 the wild type enzyme shows very low activity with glyphosate Bacillus cereus ?
-
 ?

Synonyms

Synonyms Comment Organism
GOX
-
 Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 50 the enzyme remains stable between 0 and 50°C after 6 h incubation Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 glycine mutant enzyme G51R/D60G, at pH 8.5 and 60°C Bacillus cereus
0.019
-
 glycine mutant enzyme G51R, at pH 8.5 and 60°C Bacillus cereus
0.0225
-
 glycine mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C Bacillus cereus
0.031
-
 glycine mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C Bacillus cereus
0.038
-
 glycine mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C Bacillus cereus
0.046
-
 glyphosate mutant enzyme G51R/D60G, at pH 8.5 and 60°C Bacillus cereus
0.049
-
 glyphosate mutant enzyme G51R, at pH 8.5 and 60°C Bacillus cereus
0.069
-
 glyphosate mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C Bacillus cereus
0.076
-
 glycine mutant enzyme D60G, at pH 8.5 and 60°C Bacillus cereus
0.077
-
 glyphosate mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C Bacillus cereus
0.09
-
 glycine mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C Bacillus cereus
0.09
-
 glycine mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C Bacillus cereus
0.095
-
 glycine mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C Bacillus cereus
0.095
-
 glyphosate wild type enzyme, at pH 8.5 and 60°C Bacillus cereus
0.12
-
 glyphosate mutant enzyme D60G, at pH 8.5 and 60°C Bacillus cereus
0.121
-
 glyphosate mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C Bacillus cereus
0.14
-
 glycine wild type enzyme, at pH 8.5 and 60°C Bacillus cereus
0.169
-
 glycine mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C Bacillus cereus
0.195
-
 glyphosate mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C Bacillus cereus
0.2165
-
 glyphosate mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C Bacillus cereus
0.217
-
 glycine mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C Bacillus cereus
0.22
-
 glyphosate mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C Bacillus cereus
0.513
-
 glyphosate mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C Bacillus cereus
64
-
 glyphosate mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 the enzyme remains stable between pH 6.0 and 11.0 after 6 h incubation Bacillus cereus