Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by glycine, sarcosine, and glycine ethyl ester | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.43 | - |
glycine | at pH 8.5 and 40°C | Pseudomonas putida | |
4.86 | - |
sarcosine | at pH 8.5 and 40°C | Pseudomonas putida | |
7.68 | - |
N-ethylglycine | at pH 8.5 and 40°C | Pseudomonas putida | |
31.7 | - |
D-proline | at pH 8.5 and 40°C | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + H2O + O2 | Pseudomonas putida | - |
glyoxylate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | Pseudomonas putida KT 2240 | - |
glyoxylate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88Q83 | - |
- |
Pseudomonas putida KT 2240 | Q88Q83 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | 10% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
D-alanine + H2O + O2 | 10% activity compared to glycine | Pseudomonas putida KT 2240 | ? | - |
? | |
D-proline + H2O + O2 | 25% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
D-proline + H2O + O2 | 25% activity compared to glycine | Pseudomonas putida KT 2240 | ? | - |
? | |
glycine + H2O + O2 | - |
Pseudomonas putida | glyoxylate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | - |
Pseudomonas putida KT 2240 | glyoxylate + NH3 + H2O2 | - |
? | |
glycine ethyl ester + H2O + O2 | 5% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
glycine ethyl ester + H2O + O2 | 5% activity compared to glycine | Pseudomonas putida KT 2240 | ? | - |
? | |
glycylglycine + H2O + O2 | 3% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
N-ethylglycine + H2O + O2 | 40% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
sarcosine + H2O + O2 | - |
Pseudomonas putida | ? | - |
? | |
sarcosine + H2O + O2 | - |
Pseudomonas putida KT 2240 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
ThiO | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | the enzyme is most stable in the pH range from 8.0 to 10.0 upon incubation at 25°C for 1 h | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas putida |