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Literature summary for 1.4.3.16 extracted from

  • Washio, T.; Oikawa, T.
    Thermostable and highly specific L-aspartate oxidase from Thermococcus litoralis DSM 5473 cloning, overexpression, and enzymological properties (2018), Extremophiles, 22, 59-71 .
    View publication on PubMed
Search for more literature for 1.4.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Thermococcus litoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.5
-
 L-aspartate pH 7.0, 70°C Thermococcus litoralis
10.2
-
 L-aspartate pH 7.0, 60°C Thermococcus litoralis
14.4
-
 L-aspartate pH 7.0, 50°C Thermococcus litoralis
21.1
-
 L-aspartate pH 7.0, 37°C Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 gel filtration Thermococcus litoralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + O2 Thermococcus litoralis
-
 iminosuccinate + H2O2
-
 ?
L-aspartate + O2 Thermococcus litoralis DSM 5473
-
 iminosuccinate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus litoralis H3ZLZ5
-
-
Thermococcus litoralis DSM 5473 H3ZLZ5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.86
-
 substrate: L-aspartate, pH 7.0, 70°C Thermococcus litoralis

Storage Stability

Storage Stability Organism
4°C, 96 h, the specific activity of the purified enzyme does not change during the storage period Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + O2
-
 Thermococcus litoralis iminosuccinate + H2O2
-
 ?
L-aspartate + O2 highly specific for L-aspartate and does not act on other natural amino acids Thermococcus litoralis iminosuccinate + H2O2
-
 ?
L-aspartate + O2
-
 Thermococcus litoralis DSM 5473 iminosuccinate + H2O2
-
 ?
L-aspartate + O2 highly specific for L-aspartate and does not act on other natural amino acids Thermococcus litoralis DSM 5473 iminosuccinate + H2O2
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 52000, SDS-PAGE Thermococcus litoralis

Synonyms

Synonyms Comment Organism
Tl-LASPO
-
 Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 activity increases with temperature up to 70°C Thermococcus litoralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 1 h, stable Thermococcus litoralis
85
-
 half-life time: 46 min. 1 h, approximately, 70% of its initial activity remains Thermococcus litoralis
90
-
 half-life time: 170 min Thermococcus litoralis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 L-aspartate pH 7.0, 37°C Thermococcus litoralis
1.61
-
 L-aspartate pH 7.0, 50°C Thermococcus litoralis
2.53
-
 L-aspartate pH 7.0, 60°C Thermococcus litoralis
4.26
-
 L-aspartate pH 7.0, 70°C Thermococcus litoralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermococcus litoralis

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Thermococcus litoralis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.5 highly stable between pH 5.0 and 10.5 Thermococcus litoralis

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme utilized fumaric acid, 2,6-dichlorophenolindophenol, and ferricyanide in addition to FAD as a cofactor under anaerobic conditions Thermococcus litoralis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
 L-aspartate pH 7.0, 37°C Thermococcus litoralis
0.11
-
 L-aspartate pH 7.0, 50°C Thermococcus litoralis
0.25
-
 L-aspartate pH 7.0, 60°C Thermococcus litoralis
0.57
-
 L-aspartate pH 7.0, 70°C Thermococcus litoralis