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Literature summary for 1.4.3.14 extracted from

  • Danson, J.W.; Trawick, M.L.; Cooper, A.J.
    Spectrophotometric assays for L-lysine alpha-oxidase and gamma-glutamylamine cyclotransferase (2002), Anal. Biochem., 303, 120-130.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Semicarbazide weak competitive, reversible inhibition Trichoderma viride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
L-lysine 70 mM potassium phosphate buffer pH 8.0 Trichoderma viride
0.4
-
L-lysine 100 mM potassium phosphate buffer pH 7.4 and 500 mM semicarbazide Trichoderma viride

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Trichoderma viride
-
6-amino-2-oxohexanoate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine + O2 + H2O
-
Trichoderma viride 4-methyl-2-oxopentanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O
-
Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + semicarbazide + ?
-
Trichoderma viride 4-methyl-2-oxopentanoate + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 Tris- and phosphate buffer Trichoderma viride
7.4
-
assay at Trichoderma viride

Cofactor

Cofactor Comment Organism Structure
FAD
-
Trichoderma viride

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
56
-
Semicarbazide 500 mM, 10 mM L-lysine, 3% inhibition Trichoderma viride