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Literature summary for 1.4.3.12 extracted from

  • Mirza, I.A.; Burk, D.L.; Xiong, B.; Iwaki, H.; Hasegawa, Y.; Grosse, S.; Lau, P.C.; Berghuis, A.M.
    Structural analysis of a novel cyclohexylamine oxidase from Brevibacterium oxydans IH-35A (2013), PLoS ONE, 8, e60072.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Microbacterium oxydans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 30% (w/v) PEG 8000 and 0.2 M sodium acetate trihydrate in 0.1 M sodium cacodylate pH 6.5 Microbacterium oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Microbacterium oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexylamine + O2 + H2O Microbacterium oxydans
-
cyclohexanone + NH3 + H2O2
-
?
cyclohexylamine + O2 + H2O Microbacterium oxydans IH-35A
-
cyclohexanone + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Microbacterium oxydans M5AWH1
-
-
Microbacterium oxydans IH-35A M5AWH1
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE cellulose DE-52 column chromatography, and Toyopearl Butyl-650S column chromatography Microbacterium oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexylamine + O2 + H2O
-
Microbacterium oxydans cyclohexanone + NH3 + H2O2
-
?
cyclohexylamine + O2 + H2O
-
Microbacterium oxydans IH-35A cyclohexanone + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Microbacterium oxydans

Synonyms

Synonyms Comment Organism
CHAO
-
Microbacterium oxydans