Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Microbacterium oxydans |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 30% (w/v) PEG 8000 and 0.2 M sodium acetate trihydrate in 0.1 M sodium cacodylate pH 6.5 | Microbacterium oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Microbacterium oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexylamine + O2 + H2O | Microbacterium oxydans | - |
cyclohexanone + NH3 + H2O2 | - |
? | |
cyclohexylamine + O2 + H2O | Microbacterium oxydans IH-35A | - |
cyclohexanone + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium oxydans | M5AWH1 | - |
- |
Microbacterium oxydans IH-35A | M5AWH1 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE cellulose DE-52 column chromatography, and Toyopearl Butyl-650S column chromatography | Microbacterium oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexylamine + O2 + H2O | - |
Microbacterium oxydans | cyclohexanone + NH3 + H2O2 | - |
? | |
cyclohexylamine + O2 + H2O | - |
Microbacterium oxydans IH-35A | cyclohexanone + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Microbacterium oxydans |
Synonyms | Comment | Organism |
---|---|---|
CHAO | - |
Microbacterium oxydans |