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Literature summary for 1.4.3.11 extracted from

  • Arima, J.; Tamura, T.; Kusakabe, H.; Ashiuchi, M.; Yagi, T.; Tanaka, H.; Inagaki, K.
    Recombinant expression, biochemical characterization and stabilization through proteolysis of an L-glutamate oxidase from Streptomyces sp. X-119-6 (2003), J. Biochem., 134, 805-812.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The recombinant enzyme exhibits low thermostability compared to the enzyme isolated from Streptomyces sp. X-119-6 and is an aggregated form. Proteolysis of the recombinant enzyme with metalloendopeptidase from Streptomyces griseus improves the catalytic efficiency at various pH-values and stabilizes the structure of the enzyme Streptomyces sp. X-119-6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
L-glutamate pH 6.0, 30°C, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus. pH 6.0 or 7.4, 30°C, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
5
-
L-glutamate pH 7.4, 30°C, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6

Organism

Organism UniProt Comment Textmining
Streptomyces sp. X-119-6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp. X-119-6

Storage Stability

Storage Stability Organism
4°C or -20°C, pH 7.4, stable for several month Streptomyces sp. X-119-6

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O
-
Streptomyces sp. X-119-6 2-oxoglutarate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
dimer recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
hexamer alpha2beta2gamma2,recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6

Synonyms

Synonyms Comment Organism
LGOX
-
Streptomyces sp. X-119-6

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
at pH 7.4, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
50
-
at pH 6.0, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
58
-
at pH 6.0 and at pH 7.4, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 7.4, 30 min, 50% inactivation, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
50
-
pH 6.0, 30 min, 50% inactivation, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
75
-
pH 7.4, 30 min, 50% inactivation, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
80
-
pH 6.0, 30 min, 50% inactivation, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27
-
L-glutamate pH 6.0, 30°C, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
33
-
L-glutamate pH 7.4, 30°C, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
49
-
L-glutamate pH 6.0, 30°C, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
75
-
L-glutamate pH 7.4, 30°C, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
30°C, 30 min, 50% inactivation, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6
7.4
-
50°C, 30 min, 50% inactivation, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus Streptomyces sp. X-119-6