Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The recombinant enzyme exhibits low thermostability compared to the enzyme isolated from Streptomyces sp. X-119-6 and is an aggregated form. Proteolysis of the recombinant enzyme with metalloendopeptidase from Streptomyces griseus improves the catalytic efficiency at various pH-values and stabilizes the structure of the enzyme | Streptomyces sp. X-119-6 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
L-glutamate | pH 6.0, 30°C, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus. pH 6.0 or 7.4, 30°C, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 | |
5 | - |
L-glutamate | pH 7.4, 30°C, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. X-119-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. X-119-6 |
Storage Stability | Organism |
---|---|
4°C or -20°C, pH 7.4, stable for several month | Streptomyces sp. X-119-6 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | - |
Streptomyces sp. X-119-6 | 2-oxoglutarate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
hexamer | alpha2beta2gamma2,recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
Synonyms | Comment | Organism |
---|---|---|
LGOX | - |
Streptomyces sp. X-119-6 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
at pH 7.4, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
50 | - |
at pH 6.0, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
58 | - |
at pH 6.0 and at pH 7.4, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.4, 30 min, 50% inactivation, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
50 | - |
pH 6.0, 30 min, 50% inactivation, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
75 | - |
pH 7.4, 30 min, 50% inactivation, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
80 | - |
pH 6.0, 30 min, 50% inactivation, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27 | - |
L-glutamate | pH 6.0, 30°C, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 | |
33 | - |
L-glutamate | pH 7.4, 30°C, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 | |
49 | - |
L-glutamate | pH 6.0, 30°C, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 | |
75 | - |
L-glutamate | pH 7.4, 30°C, recombinant enzyme treated with metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
30°C, 30 min, 50% inactivation, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |
7.4 | - |
50°C, 30 min, 50% inactivation, recombinant enzyme before proteolysis by metalloendopeptidase from Streptomyces griseus | Streptomyces sp. X-119-6 |