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Literature summary for 1.4.3.10 extracted from

  • van Hellemond, E.W.; van Dijk, M.; Heuts, D.P.; Janssen, D.B.; Fraaije, M.W.
    Discovery and characterization of a putrescine oxidase from Rhodococcus erythropolis NCIMB 11540 (2008), Appl. Microbiol. Biotechnol., 78, 455-463.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Rhodococcus erythropolis

Protein Variants

Protein Variants Comment Organism
E324A the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme Rhodococcus erythropolis
E324L the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme Rhodococcus erythropolis

Inhibitors

Inhibitors Comment Organism Structure
(2-aminoethyl)-trimethylammonium competitive inhibitor Rhodococcus erythropolis
1,3-diaminopropane competitive inhibitor Rhodococcus erythropolis
Aminoethanol competitive inhibitor Rhodococcus erythropolis
ethylenediamine competitive inhibitor Rhodococcus erythropolis
additional information short diamines and monoamines strongly inhibit activity Rhodococcus erythropolis
n-butylamine competitive inhibitor Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
putrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
0.0082
-
putrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.018
-
cadaverine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.065
-
2-hydroxyputrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.1
-
spermidine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.17
-
1,6-diaminohexane wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.33
-
L-ornithine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.47
-
spermine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
110
-
putrescine mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
110
-
putrescine mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Rhodococcus erythropolis
98750
-
theoretical molecular mass Rhodococcus erythropolis
108000
-
SDS-PAGE Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis B0F9F6 fragment
-
Rhodococcus erythropolis NCIMB 11540 B0F9F6 fragment
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,6-diaminohexane + O2 + H2O 1.4% activity compared to putrescine Rhodococcus erythropolis 6-aminohexanal + NH3 + H2O2
-
?
2-hydroxyputrescine + O2 + H2O 28% activity compared to putrescine Rhodococcus erythropolis ?
-
?
4-amino-1-butanol + O2 + H2O 0.6% activity compared to putrescine Rhodococcus erythropolis 4-hydroxybutanal + NH3 + H2O2
-
?
cadaverine + O2 + H2O 14% activity compared to putrescine Rhodococcus erythropolis 5-aminopentanal + NH3 + H2O2
-
?
cadaverine + O2 + H2O 14% activity compared to putrescine Rhodococcus erythropolis NCIMB 11540 5-aminopentanal + NH3 + H2O2
-
?
L-ornithine + O2 + H2O 0.08% activity compared to putrescine Rhodococcus erythropolis ?
-
?
additional information no activity with aromatic amines and amino alcohols Rhodococcus erythropolis ?
-
?
additional information no activity with aromatic amines and amino alcohols Rhodococcus erythropolis NCIMB 11540 ?
-
?
putrescine + O2 + H2O 100% activity Rhodococcus erythropolis 4-aminobutanal + NH3 + H2O2
-
?
putrescine + O2 + H2O 100% activity Rhodococcus erythropolis NCIMB 11540 4-aminobutanal + NH3 + H2O2
-
?
spermidine + O2 + H2O 5.3% activity compared to putrescine Rhodococcus erythropolis 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
?
spermidine + O2 + H2O 5.3% activity compared to putrescine Rhodococcus erythropolis NCIMB 11540 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
?
spermine + O2 + H2O 0.7% activity compared to putrescine Rhodococcus erythropolis N,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2
-
?
spermine + O2 + H2O 0.7% activity compared to putrescine Rhodococcus erythropolis NCIMB 11540 N,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 54000, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
PuO
-
Rhodococcus erythropolis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 50 at 50°C, half of the activity is lost after 2 h, while at 37°C, such a degree of inactivation is reached after 1 day Rhodococcus erythropolis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0198
-
L-ornithine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.18
-
spermine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.37
-
1,6-diaminohexane wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
1.4
-
spermidine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
3.2
-
putrescine mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
3.9
-
cadaverine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
6.1
-
putrescine mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
7.5
-
2-hydroxyputrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
20.7
-
putrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C Rhodococcus erythropolis
26.4
-
putrescine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
around pH 8.0 Rhodococcus erythropolis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.4
-
below pH 6.4, no significant activity can be detected Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
FAD contains non-covalently bound FAD Rhodococcus erythropolis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0018
-
Aminoethanol wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.064
-
1,3-diaminopropane wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.12
-
n-butylamine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
0.66
-
ethylenediamine wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis
5.8
-
(2-aminoethyl)-trimethylammonium wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Rhodococcus erythropolis